A highly sensitive method was developed to measure putrescine by micellar electrokinetic chromatography with laser induced fluorescence detection with excellent linearity in the 1 nM to 3 μM range. The technique was tested on a drop of blood from Parkinson's disease patients obtained by finger prick. The results showed a statistically significant increase of putrescine in the erythrocytes compared to controls and a non-significant increase in plasma. This high level of putrescine does not constitute by itself proof that putrescine and polyamines are directly related to Parkinson's disease. However, the present results and several others addressed in the discussion suggest that these compounds might be causally involved in the pathophysiology of Parkinson's disease. In addition, the analytical method reported here may help to find new biomarkers for many diseases including Parkinson's disease.

开发了一种高度敏感的方法，可通过胶束电动色谱与激光诱导的荧光检测在1 nM至3μM范围内具有出色的线性度来测定腐胺。该技术在通过手指刺刺获得的帕金森氏病患者的一滴血液上进行了测试。结果显示，与对照组相比，红细胞中的腐胺具有统计学上的显着增加，血浆中无显着增加。这种高水平的腐胺本身并不能证明腐胺和多胺与帕金森氏病直接相关。但是，目前的结果以及讨论中讨论的其他一些结果表明，这些化合物可能与帕金森氏病的病理生理学有因果关系。此外，