Lantibiotics are ribosomally synthesized and post-translationally modified peptide natural products that contain thioether cross-links formed by lanthionine and methyllanthionine residues. They exert potent antimicrobial activity against Gram-positive bacteria. We herein report production of analogues of two lantibiotics, lacticin 481 and nisin, that contain nonproteinogenic amino acids using two different strategies involving amber stop codon suppression technology. These methods complement recent alternative approaches to incorporate nonproteinogenic amino acids into lantibiotics.

中文翻译：

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非蛋白氨基酸掺入I和II类羊毛硫抗生素中

羊毛硫抗生素是核糖体合成和翻译后修饰的肽天然产物，其包含由羊毛硫氨酸和甲基羊毛硫氨酸残基形成的硫醚交联。它们对革兰氏阳性细菌发挥有效的抗菌活性。我们在此报告了使用两种涉及琥珀色终止密码子抑制技术的不同策略生产的两种羊毛硫抗生素的类似物，乳酸菌素481和乳链菌肽，它们含有非蛋白原性氨基酸。这些方法补充了将非蛋白氨基酸掺入羊毛硫抗生素的最新替代方法。