Gold nanoparticles (Au NPs) have been confirmed to show excellent photothermal conversion property for tumor theranostic applications. To improve the antitumor efficacy, a novel nanoplatform system composed of porphyrin derivative and Au NPs was fabricated to study the dual-modality photodynamic and photothermal therapy with laser irradiation. Modified chitosan was coated on the Au NPs surface via ligand exchange between thiol groups and Au. The chitosan-coated Au NPs (QCS-SH/Au NPs) were further conjugated with meso-tetrakis(4-sulphonatophenyl)porphyrin (TPPS) via electrostatic interaction to obtain the porphyrin-conjugated Au hybrid nanoparticles (TPPS/QCS-SH/Au NPs). Size, morphology, and properties of the prepared nanoparticles were confirmed by Zeta potential, nanoparticle size analyzer, transmission electron microscopy (TEM), and UV–vis spectroscopy. Moreover, both photothermal therapy (PTT) and photodynamic therapy (PDT) were investigated. Compared with alone Au NPs or TPPS, the hybrid TPPS/QCS-SH/Au NPs with lower cytotoxicity showed durable elevated temperature to around 56 °C and large amount of singlet oxygen (¹O₂) produced from TPPS. Thus, the hybrid nanoparticles showed a more significant synergistic therapy effect of hyperthermia from PTT as well as ¹O₂ from PDT, which has potential applications in the tumor therapy fields.

中文翻译：

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卟啉衍生物与金纳米粒子结合的双模态光动力和光热疗法的体外。

金纳米颗粒（Au NPs）已被证实在肿瘤治疗应用中显示出优异的光热转化性能。为了提高抗肿瘤功效，制备了由卟啉衍生物和金纳米颗粒组成的新型纳米平台系统，以研究激光照射的双模态光动力和光热疗法。通过硫醇基团与Au之间的配体交换将改性的壳聚糖涂覆在Au NPs表面上。通过静电相互作用将壳聚糖包被的金纳米颗粒（QCS-SH / Au NPs）与内四（4-磺基苯基）卟啉（TPPS）进一步偶联，得到卟啉偶联的金杂化纳米颗粒（TPPS / QCS-SH / Au） NPs）。通过Zeta电势，纳米粒度分析仪，透射电子显微镜（TEM）确认了所制备纳米颗粒的尺寸，形态和性能。和紫外可见光谱。此外，还研究了光热疗法（PTT）和光动力疗法（PDT）。与单独的Au NPs或TPPS相比，具有较低细胞毒性的TPPS / QCS-SH / Au杂交NPs具有持久的高温，可升高至56°C左右，并具有大量的单重态氧（TPPS产生¹ O ₂）。因此，杂化纳米粒子表现出更显着的PTT热疗和PDT ¹ O ₂协同治疗效果，在肿瘤治疗领域具有潜在的应用前景。