Cyclodextrin enhanced pulsed ultrasound assisted extraction procedure was developed for obtaining olive pomace extract rich in bioactive polyphenols, principally oleuropein, tyrosol, and hydroxytyrosol. 20-minute extraction using 12 mm probe at 100% output intensity was selected as optimal for the extraction of olive pomace polyphenols, using 60% ethanol at 1:40 solvent to solid ratio. Addition of cyclodextrins to extraction solvent (especially randomly-methylatedbeta cyclodextrin and hydroxypropyl-beta cyclodextrin) significantly affected extraction yields, stability and the appearance (colour and particle size distribution) of obtained extracts. Extracts obtained under optimized conditions contained 887 mgkg-1 of hydroxytyrosol, 1117 mgkg-1 of tyrosol and 1744 mgkg-1 of oleuropein. In comparison to conventional solvent extraction, application of ultrasound-assisted extraction resulted in significantly higher yields, reduced extraction time and solvent consumption.

开发了环糊精增强脉冲超声辅助提取程序，以获得富含生物活性多酚（主要是橄榄苦苷，酪醇和羟基酪醇）的橄榄果渣提取物。选择使用100 mm输出强度的12 mm探针进行20分钟的提取是提取橄榄果渣多酚的最佳方法，使用60％乙醇以1:40的溶剂/固体比率进行提取。将环糊精添加到提取溶剂（尤其是无规甲基化的β-环糊精和羟丙基-β-环糊精）中会显着影响提取物的收率，稳定性和外观（颜色和粒度分布）。在优化条件下获得的提取物含有887 mgkg-1的羟基酪醇，1117 mgkg-1的酪醇和1744 mgkg-1的橄榄苦苷。与传统的溶剂萃取相比，