LWT - Food Science and Technology ( IF 6 ) Pub Date : 2018-02-10 , DOI: 10.1016/j.lwt.2018.02.023 Fariba Moghaddasi , Mohammad Reza Housaindokht , Majid Darroudi , Mohammad Reza Bozorgmehr , Abbas Sadeghi
Curcumin is an important biologically active polyphenolic compound that has been found to contain the ability of a wide range of beneficial biological activities. The aim of this study was to develop a method for the preparation of curcumin nanoparticles (Nano-CUR) with a view to improve its aqueous-phase solubility and examine the effect on its biological properties. Nano-CUR were prepared in black pepper oil (BP oil) as an oil phase and Tween 80 as the surfactant. Nano-CUR was prepared by a modified microemulsion technique and was found to have a narrow particle size distribution in the ranges of 8–26 nm. Nano-CUR was obtained with a spherical shape and mean particle size of 15.7 ± 3.55 nm when the following optimal conditions were adopted as water/oil (5:1) and oil/surfactant (1:9). Unlike curcumin (bulk), Nano-CUR is readily dispersed in aqueous media. The FTIR spectrum indices can be used in order to differentiate nano and non-nano emulsion solutions. Antioxidant activity tests were applied to the Emulsion components, Nano-CUR, and also to Tween 80 + BP oil. Results demonstrated that Nano-CUR has maximum antioxidant activity. In vitro cytotoxicity effect of Nano-CUR was also investigated, which was detected in the treated Neuro2A cells to be up to 15.6 μg/mL.
中文翻译:
O / W纳米乳液技术利用黑胡椒油合成纳米姜黄素及其生物学活性研究
姜黄素是一种重要的生物活性多酚化合物,已发现其具有多种有益生物活性的能力。这项研究的目的是开发一种制备姜黄素纳米颗粒(Nano-CUR)的方法,以改善其水相溶解度并研究对其生物学特性的影响。Nano-CUR在黑胡椒油(BP油)中作为油相,在Tween 80中作为表面活性剂制备。Nano-CUR是通过改进的微乳液技术制备的,发现在8–26 nm范围内具有较窄的粒径分布。当采用以下最佳条件作为水/油(5:1)和油/表面活性剂(1:9)时,获得的纳米CUR具有球形形状,平均粒径为15.7±3.55 nm。与姜黄素(散装)不同,纳米-CUR容易分散在水性介质中。可以使用FTIR光谱指数来区分纳米和非纳米乳液溶液。将抗氧化活性测试应用于乳液成分Nano-CUR,以及吐温80 + BP油。结果表明,Nano-CUR具有最大的抗氧化活性。还研究了Nano-CUR的体外细胞毒性作用,在处理的Neuro2A细胞中检测到的毒性高达15.6μg/ mL。