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Protein phosphatase 4 mediates palmitic acid-induced endothelial dysfunction by decreasing eNOS phosphorylation at serine 633 in HUVECs Exp. Cell Res. (IF 3.7) Pub Date : 2024-03-20 Zhengwei Liang, Gang Sun, Junshi Zhang, Qian Zhang, Xiaoyu Li, Si Qin, Sha Lv, Jing Ding, Qifang Zhang, Yong Xia, Deqin Lu
Plasma saturated free fatty acid (FFA)-induced endothelial dysfunction (ED) contributes to the pathogenesis of atherosclerosis and cardiovascular diseases. However, the mechanism underlying saturated FFA-induced ED remains unclear. This study demonstrated that palmitic acid (PA) induced ED by activating the NADPH oxidase (NOX)/ROS signaling pathway to activate protein phosphatase 4 (PP4) and protein
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TMEM158 functions as an oncogene and promotes lung adenocarcinoma progression through the PI3K/AKT pathway via interaction with TWIST1 Exp. Cell Res. (IF 3.7) Pub Date : 2024-03-19 Tao Xu, Fang Yin, Kaihu Shi
Lung adenocarcinoma (LUAD) is a common and deadly form of lung cancer, with high rates of metastasis and unsatisfactory clinical outcomes. Herein, we examined the influence of TMEM158 on the LUAD progression. A combination of bioinformatic analyses was used to assess the TMEM158 expression pattern, prognostic implications, and potential function in LUAD. The levels of TMEM158 and TWIST1 were evaluated
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TGF-α/EGFR signaling promotes lipopolysaccharide-induced abnormal elastin deposition and alveolar simplification Exp. Cell Res. (IF 3.7) Pub Date : 2024-03-19 Jianhui Li, Jian Cao, Chongbing Yan, Xiaohui Gong
Bronchopulmonary dysplasia (BPD) is characterized by shortened secondary septa and fewer, larger alveoli. Elastin deposition to the distal tips of the secondary septa is critical for elongation of the secondary septa. Alveolar myofibroblasts, which are thought to migrate to the septal tips during alveolarization, are mainly responsible for elastin production and deposition. Antenatal exposure to inflammation
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Inhibition of PD-L1 expression in non-small cell lung cancer may reduce vasculogenic mimicry formation by inhibiting the epithelial mesenchymal transformation process Exp. Cell Res. (IF 3.7) Pub Date : 2024-03-18 Yuqi Shi, Wenjuan Li, Qianhao Jia, Jiatao Wu, Shoufan Wu, Shiwu Wu
Non-small cell lung cancer (NSCLC) is a kind of highly malignant tumor. Studies have shown that Vasculogenic mimicry (VM) may be responsible for dismal prognosis in NSCLC. Immunotherapy with programmed death-1 (PD-1) or programmed death ligand-1 (PD-L1) has significantly altered the treatment of assorted cancers, including NSCLC, but its role and mechanism in the formation of Vasculogenic mimicry (VM)
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A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro Exp. Cell Res. (IF 3.7) Pub Date : 2024-03-16 Flavio Bonanini, Madhulika Singh, Hong Yang, Dorota Kurek, Amy C. Harms, Adil Mardinoglu, Thomas Hankemeier
Hepatocytes are responsible for maintaining a stable blood glucose concentration during periods of nutrient scarcity. The breakdown of glycogen and synthesis of glucose are crucial metabolic pathways deeply interlinked with lipid metabolism. Alterations in these pathways are often associated with metabolic diseases with serious clinical implications. Studying energy metabolism in human cells is challenging
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KLF10/CBS increases the sensitivity of gastric carcinoma cells to methionine restriction by promoting sulfur transfer pathway Exp. Cell Res. (IF 3.7) Pub Date : 2024-03-16 Lin Xin, Zhi-Yang Liu, Chen-Xi Liu, Jie Sheng, Qi Zhou, Yi-wu Yuan, Zhen-Qi Yue, Hao Lu, Jin-Heng Gan
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Regulation of the AGEs-induced inflammatory response in human periodontal ligament cells via the AMPK/NF-κB/ NLRP3 signaling pathway Exp. Cell Res. (IF 3.7) Pub Date : 2024-03-16 Zhu-Ling Guo, Jie Zhou, Xue-Jing Lin, Qing Yuan, Yu-Lei Dong, Qi-Bing Liu, Tao Wang
The heightened prevalence and accelerated progression of periodontitis in individuals with diabetes is primarily attributed to inflammatory responses in human periodontal ligament cells (HPDLCs). This study is aimed at delineating the regulatory mechanism of nucleotide-binding oligomerization domain-like receptors (NLRs) in mediating inflammation incited by muramyl dipeptide (MDP) in HPDLCs, under
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Notoginsenoside R1 targets PPAR-γ to inhibit hepatic stellate cell activation and ameliorates liver fibrosis Exp. Cell Res. (IF 3.7) Pub Date : 2024-03-16 Cheng Guo, Linying Lai, Boyu Ma, Qian Huang, Zhirong Wang
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Targeting inhibitor of apoptosis proteins (IAPs) enhances susceptibility of oral squamous carcinoma cells to cisplatin Exp. Cell Res. (IF 3.7) Pub Date : 2024-03-13 Tianyi Gao, Stefania Magnano, Amy Rynne, Lucy O'Kane, Patricia Hannon Barroeta, Daniela M. Zisterer
Oral Squamous Cell Carcinoma (OSCC) is the 6th most common cancer worldwide. It is generally aggressive and closely associated with chemoresistance and poor survival. There is accumulating evidence for the involvement of inhibitors of apoptosis proteins (IAPs), including IAP1 and XIAP, in mediating chemotherapy resistance in OSCC. Various strategies for targeting IAPs have been designed and tested
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Single-cell tracking as a tool for studying EMT-phenotypes Exp. Cell Res. (IF 3.7) Pub Date : 2024-03-13 Ellen Marie Botne Quinsgaard, Mónica Suárez Korsnes, Reinert Korsnes, Siver Andreas Moestue
This article demonstrates that label-free single-cell video tracking is a useful approach for studies of Epithelial-Mesenchymal Transition (EMT). EMT is a highly heterogeneous process, involved in wound healing, embryogenesis and cancer. The process promotes metastasis, and increased understanding can aid development of novel therapeutic strategies. The role of EMT-associated biomarkers depends on
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ALKBH5-mediated upregulation of CPT1A promotes macrophage fatty acid metabolism and M2 macrophage polarization, facilitating malignant progression of colorectal cancer Exp. Cell Res. (IF 3.7) Pub Date : 2024-03-12 Mingming Sun, Yinzi Yue, Xiaopeng Wang, Huayi Feng, Yuanyuan Qin, Mengyao Chen, Yahui Wang, Shuai Yan
m6A modification has been studied in tumors, but its role in host anti-tumor immune response and TAMs polarization remains unclear. The fatty acid oxidation (FAO) process of TAMs is also attracting attention.
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METTL3 promotes trophoblast ferroptosis in preeclampsia by stabilizing the ACSL4 m6A modification Exp. Cell Res. (IF 3.7) Pub Date : 2024-03-09 Yang Wang, Gang Zhang, Yan Gao, Xuemei Zhang, Hongbo Qi
This study aims to explore the role of methyltransferase-like 3 (METTL3) modulation of ferroptosis in the pathogenesis of trophoblast-mediated preeclampsia. The expression of METTL3 and acyl-CoA synthetase long chain family member 4 (ACSL4) was measured in clinical placental tissues and trophoblasts using qPCR and Western blot techniques. The effects of METTL3 on the symptoms of preeclampsia were also
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The KDM5 inhibitor PBIT reduces proliferation of castration-resistant prostate cancer cells via cell cycle arrest and the induction of senescence Exp. Cell Res. (IF 3.7) Pub Date : 2024-03-08 Tunde Smith, Tytianna White, Zhenbang Chen, LaMonica V. Stewart
The compound 2–4(4-methylphenyl)-1,2-benzisothiazol-3(2H)-one (PBIT) is an inhibitor of the KDM5 family of lysine-specific histone demethylases that has been suggested as a lead compound for cancer therapy. The goal of this study was to explore the effects of PBIT within human prostate cancers. Micromolar concentrations of PBIT altered proliferation of castration-sensitive LNCaP and castration-resistant
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High level 27-HC impairs trophoblast cell invasion and migration via LXR in pre-eclampsia Exp. Cell Res. (IF 3.7) Pub Date : 2024-03-08 Hai Tong, Tingting Wang, Ruixin Chen, Pingsong Jin, Youqing Jiang, Xue Zhang, Hongbo Qi
To explore the potential impact of 27-hydroxycholesterol (27-HC) on trophoblast cell function in pre-eclampsia. The levels of 27-HC and the expression of CYP27A1 are upregulated in clinical samples of PE. Furthermore, high concentrations of 27-HC can inhibit the invasion and migration ability of trophoblast cells in vitro, and this inhibitory effect is weakened after LXR silencing. In HTR8/SVneo cells
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Corrigendum to TWIST2 inhibits EMT and induces oxidative stress of lung cancer cells by regulating FGF21-mediated AMPK/mTOR pathway [Exp. Cell Res. 2021 Aug 1;405(1):112661/ECR-21-80] Exp. Cell Res. (IF 3.7) Pub Date : 2024-03-02 Yingjian Song, Wei Zhang, Jiuxu Zhang, Zhaolei You, Tao Hu, Guangyuan Shao, Zheng Zhang, Zhicheng Xu, Xiaofeng Yu
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Rab25 is involved in hypospadias via the β1 integrin/EGFR pathway Exp. Cell Res. (IF 3.7) Pub Date : 2024-02-23 Zhicheng Zhang, Qiang Zhang, Zhenmin Liu, Chong Wang, Hongsong Chen, Xingguo Luo, Lianju Shen, Chunlan long, Guanghui Wei, Xing Liu
Hypospadias is a common congenital abnormality of the penile. Abnormal regulation of critical genes involved in urethral development leads to hypospadias. We used the Rab25 mice and foreskin fibroblasts transfected with lentivirus in vitro and in vivo to investigate the role of Rab25 in hypospadias. The expression levels of various molecules in tissue samples and foreskin fibroblasts were confirmed
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Anti-proliferation and apoptosis induced via the mTOR/PGC-1α signaling pathway in trophoblast cells of miscarriage Exp. Cell Res. (IF 3.7) Pub Date : 2024-02-22 Yuanchang Zhu, Feng Wang, Zhuanghong Ma, Sumei Hou, Weifen Deng, Yaou Zhang, Qiongfang Wu
Miscarriage is a common complication during early pregnancy and affects approximately 10%–15% of all pregnant women. Several studies have reported that the abnormal expression of mitochondrial oxidative stress-related genes might be involved in the occurrence and progression of miscarriage. The present study attempted to uncover the role of peroxisome proliferator-activated receptor gamma coactivator-1α
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ICBP90, an epigenetic regulator, induces DKK3 promoter methylation, promotes glioma progression, and reduces sensitivity to cis-platinum Exp. Cell Res. (IF 3.7) Pub Date : 2024-02-22 Qinchen Cao, Xinxin Wang, Jie Liu, Yang Dong, Xiaolong Wu, Yin Mi, Ke Liu, Mingzhi Zhang, Yonggang Shi, Ruitai Fan
Glioma is the most common brain malignancy, characterized by high morbidity, high mortality, and treatment-resistance. Inverted CCAAT box Binding Protein of 90 kDa (ICBP90) has been reported to be involved in tumor progression and the maintenance of DNA methylation. Herein, we constructed ICBP90 over-expression and knockdown glioma cell lines, and found that ICBP90 knockdown inhibited glioma cell proliferation
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Synovia tissue-specific exosomes participate in the dual variation of the osteoarthritis microenvironment via miR-182 Exp. Cell Res. (IF 3.7) Pub Date : 2024-02-20 Shiqiang Wu, Jun Luo, Xiaolu Zhang, Liangmin Wang, Liquan Cai, Jie Xu
Osteoarthritis (OA) is the most common type of joint disease and the leading cause of chronic disability among older adults. As an important component of the joint, synovium influences the inflammatory and degenerative process of OA. This study found that miRNA 182 (miR-182) in synovium-specific exosomes can modulate inflammation and apoptotic signaling. It also regulated different biological functions
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RANK/RANKL axis promotes migration, invasion, and metastasis of osteosarcoma via activating NF-κB pathway Exp. Cell Res. (IF 3.7) Pub Date : 2024-02-19 Tomoya Takeda, Masanobu Tsubaki, Shuji Genno, Kana Tomita, Shozo Nishida
Osteosarcoma (OS) is one of the most prevalent primary bone tumors with a high degree of metastasis and poor prognosis. Epithelial-to-mesenchymal transition (EMT) is a cellular mechanism that contributes to the invasion and metastasis of cancer cells, and OS cells have been reported to exhibit EMT-like characteristics. Our previous studies have shown that the interaction between tumor necrosis factor
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Tumor-derived exosomal hyaluronidase 1 induced M2 macrophage polarization and promoted esophageal cancer progression Exp. Cell Res. (IF 3.7) Pub Date : 2024-02-19 Jinyan Yuan, Bin Hou, Kai Guo, Jianfei Zhu, Haimin Xiao
The communication between tumor-derived exosomes and macrophages plays an important role in facilitating the progression of tumors. However, the regulatory mechanisms by which exosomes regulate tumor progression in esophageal squamous cell carcinoma (ESCC) have not been fully elucidated. We constructed a coculture system containing an ESCC cell line and macrophages using a Transwell chamber. We isolated
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The loss of antioxidant activities impairs intestinal epithelium homeostasis by altering lipid metabolism Exp. Cell Res. (IF 3.7) Pub Date : 2024-02-18 Javier Ramos-León, Concepción Valencia, Mariana Gutiérrez-Mariscal, David-Alejandro Rivera-Miranda, Celina García-Meléndrez, Luis Covarrubias
Reactive oxygens species (ROS) are common byproducts of metabolic reactions and could be at the origin of many diseases of the elderly. Here we investigated the role of ROS in the renewal of the intestinal epithelium in mice lacking catalase (CAT) and/or nicotinamide nucleotide transhydrogenase (NNT) activities. mice have delayed intestinal epithelium renewal and were prone to develop necrotizing enterocolitis
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Upregulation of serine metabolism enzyme PSAT1 predicts poor prognosis and promotes proliferation, metastasis and drug resistance of clear cell renal cell carcinoma Exp. Cell Res. (IF 3.7) Pub Date : 2024-02-17 Jiali Ye, Xing Huang, Shuo Tian, Jichen Wang, Hanfeng Wang, Huayi Feng, Xupeng Zhao, Shouqing Cao, Yundong Xuan, Xiubin Li, Xin Ma, Yan Huang, Xu Zhang
Serine metabolic reprogramming is known to be associated with oncogenesis and tumor development. The key metabolic enzyme PSAT1 has been identified as a potential prognostic marker for various cancers, but its role in ccRCC remains unkown. In this study, we investigated expression of PSAT1 in ccRCC using the TCGA database and clinical specimens. Our results showed that PSAT1 exhibited lower expression
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Loss-of-function of kinesin-5 KIF11 causes microcephaly, chorioretinopathy, and developmental disorders through chromosome instability and cell cycle arrest Exp. Cell Res. (IF 3.7) Pub Date : 2024-02-16 Yi Zhou, Meng-Fei Xu, Jie Chen, Jing-Lian Zhang, Xin-Yao Wang, Min-Hui Huang, Ya-Lan Wei, Zhen-Yu She
Kinesin motors play a fundamental role in development by controlling intracellular transport, spindle assembly, and microtubule organization. In humans, patients carrying mutations in KIF11 suffer from an autosomal dominant inheritable disease called microcephaly with or without chorioretinopathy, lymphoedema, or mental retardation (MCLMR). While mitotic functions of KIF11 proteins have been well documented
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Increased extracellular matrix stiffness regulates myofibroblast transformation through induction of autophagy-mediated Kindlin-2 cytoplasmic translocation Exp. Cell Res. (IF 3.7) Pub Date : 2024-02-10 Zhihan Zhao, Wuyue Han, Guotao Huang, Yong He, Xiaohu Zuo, Li Hong
The extracellular matrix (ECM) mechanical properties regulate biological processes, such as fibroblast-myofibroblast transformation (FMT), which is a crucial component in pelvic organ prolapse (POP) development. The ‘Kindlin-2’ protein, expressed by fibroblasts, plays an important role in the development of the mesoderm, which is responsible for connective tissue formation; however, the role of Kindlin-2
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scaRNA20 promotes pseudouridylatory modification of small nuclear snRNA U12 and improves cardiomyogenesis Exp. Cell Res. (IF 3.7) Pub Date : 2024-02-09 Selene Perales, Vinoth Sigamani, Sheeja Rajasingh, Narasimman Gurusamy, Douglas Bittel, Andras Czirok, Marko Radic, Johnson Rajasingh
Non-coding RNAs, particularly small Cajal-body associated RNAs (scaRNAs), play a significant role in spliceosomal RNA modifications. While their involvement in ischemic myocardium regeneration is known, their role in cardiac development is unexplored. We investigated scaRNA20's role in iPSC differentiation into cardiomyocytes (iCMCs) via overexpression and knockdown assays. We measured scaRNA20-OE-iCMCs
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Inhibition of RhoGEF/RhoA alleviates regorafenib resistance and cancer stemness via Hippo signaling pathway in hepatocellular carcinoma Exp. Cell Res. (IF 3.7) Pub Date : 2024-02-08 He-Ming Zhou, Da-Hong Chen, Wen-Jing Diao, Ya-Fei Wu, Ji-Gang Zhang, Lin Zhong, Zhong-Yi Jiang, Xue Zhang, Gao-Lin Liu, Qin Li
Patients with hepatocellular carcinoma (HCC) are vulnerable to drug resistance. Although drug resistance has been taken much attention to HCC therapy, little is known of regorafenib and regorafenib resistance (RR). This study aimed to determine the drug resistance pattern and the role of RhoA in RR. Two regorafenib-resistant cell lines were constructed based on Huh7 and Hep3B cell lines. In vitro and
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MARCH-I: A negative regulator of dendritic cell maturation Exp. Cell Res. (IF 3.7) Pub Date : 2024-02-07 Maryam Valizadeh, Reza Raoofian, Afrooz Homayoonfar, Esmerdis Hajati, Ali A. Pourfathollah
The expression of costimulatory molecules such as MHC-II, CD86 and CD83 on dendritic cells (DCs) are strongly regulated during cellular activation. Ubiquitination of some of these markers by the E3 ubiquitin ligase MARCH-I affects the maturation state of DCs and subsequently modulates immune responses. The effects of MARCH-I gene overexpression on the functional activity of human DCs is not well understood
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Amyloid β interferes with wound healing of brain microvascular endothelial cells by disorganizing the actin cytoskeleton Exp. Cell Res. (IF 3.7) Pub Date : 2024-02-06 Takuma Maeda, Keiya Shimamori, Hiroki Kurita, Kiyotaka Tokuraku, Masahiro Kuragano
Cerebral amyloid angiopathy (CAA) is a disease in which amyloid β (Aβ) is deposited in the cerebral blood vessels, reducing compliance, tearing and weakening of vessels’ walls, leading to cerebral hemorrhage. The mechanisms by which Aβ leads to focal wall fragmentation and intimal damage are not well understood. We analyzed the motility of human brain microvascular endothelial cells (hBMECs) in real-time
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Exosomes derived from human umbilical cord mesenchymal stem cells pretreated by monosialoteterahexosyl ganglioside alleviate intracerebral hemorrhage by down-regulating autophagy Exp. Cell Res. (IF 3.7) Pub Date : 2024-02-03 Dongdong Yan, Yunpeng Shi, Chengrui Nan, Qianxu Jin, Yayu Zhuo, Haoran Huo, Shiqi Kong, Zongmao Zhao
Intracerebral hemorrhage (ICH) results in substantial morbidity, mortality, and disability. Depleting neural cells in advanced stages of ICH poses a significant challenge to recovery. The objective of our research is to investigate the potential advantages and underlying mechanism of exosomes obtained from human umbilical cord mesenchymal stem cells (hUMSCs) pretreated with monosialoteterahexosyl ganglioside
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Acidity-induced ITGB6 promote migration and invasion of lung cancer cells by epithelial-mesenchymal transition and focal adhesion Exp. Cell Res. (IF 3.7) Pub Date : 2024-02-03 Linxin Liu, Zhuoru He, Zhangyu Jiang, Zhongqiu Liu, Xiaojun Zhuang
Non-small cell lung cancer (NSCLC) is a prevalent tumor and acidic tumor microenvironment provides an energy source driving tumor progression. We previously demonstrated significantly upregulated Integrin β6 (ITGB6) in NSCLC cells. This study was designed to investigate the role of ITGB6 in NSCLC metastasis and explore the potential mechanisms. The expression of ITGB6 was evaluated in patients with
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Cancer-associated fibroblasts contributed to hepatocellular carcinoma recurrence and metastasis via CD36-mediated fatty-acid metabolic reprogramming Exp. Cell Res. (IF 3.7) Pub Date : 2024-01-30 Han Wang, Fangming Liu, Xiaoling Wu, Guiqi Zhu, Zheng Tang, Weifeng Qu, Qianfu Zhao, Run Huang, Mengxin Tian, Yuan Fang, Xifei Jiang, Chenyang Tao, Jun Gao, Weiren Liu, Jian Zhou, Jia Fan, Duojiao Wu, Yinghong Shi
Cancer-associated fibroblasts (CAFs) are the main components in the tumor microenvironment. Tumors activate fibroblasts from quiescent state into activated state by secreting cytokines, and activated CAFs may in turn promote tumor progression and metastasis. Therefore, studies targeting CAFs could enrich the therapeutic options for tumor treatment. In this study, we demonstrate that the content of
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Plin2 inhibits autophagy via activating AKT/mTOR pathway in non-small cell lung cancer Exp. Cell Res. (IF 3.7) Pub Date : 2024-01-30 Yawei Wang, Ye Hu, Rongjian Xu, Xiangfeng Jin, Wenjie Jiao
Perilipin 2 (Plin2) is known to be dysregulated in several human malignancies, which facilitates cancer progression. Recent studies have found that the abnormal expression of Plin2 is associated with poor prognosis of non-small cell lung cancer (NSCLC). However, the specific role of Plin2 and its underlying mechanism remain unclear. This study revealed that Plin2 expression was low in NSCLC tissues
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The competitive mechanism of EZH1 and EZH2 in promoting oral squamous cell carcinoma Exp. Cell Res. (IF 3.7) Pub Date : 2024-02-02 Jianghai Chen, Shanshan Tang, Qiuhan Zheng, Jingyuan Li, Hong Jiang, Huanzi Lu, Guiqing Liao, Kan Li, Yujie Liang
Enhancer of Zeste Homolog 1 (EZH1) and Enhancer of Zeste Homolog 2 (EZH2) are the key components of polycomb repressive complex 2 (PRC2); however, the roles of these proteins in oral squamous cell carcinoma (OSCC) have yet to be elucidated. In this study, we aimed to determine the respective roles of these proteins in OSCC by investigating the expression levels of EZH1 and EZH2 in OSCC tissues (N =
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Inhibition of vinculin activity has an adverse effect on porcine ovarian cells Exp. Cell Res. (IF 3.7) Pub Date : 2024-02-02 Barbora Loncová, Zuzana Fabová, Vladimíra Mondočková, Radoslav Omelka, Abdel Halim Harrath, Alexander V. Sirotkin
The existing knowledge of the involvement of vinculin (VCL) in the control of ovarian cell functions is insufficient. To understand the role of VCL in the control of basic porcine ovarian granulosa cell functions, we decreased VCL activity by small interfering RNA (VCL siRNA). The expression of VCL, accumulation of VCL protein, cell viability, proliferation (accumulation of PCNA and cyclin B1), proportion
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Identification of necroptosis-associated miRNA signature for predicting prognosis and immune landscape in stomach adenocarcinoma Exp. Cell Res. (IF 3.7) Pub Date : 2024-02-02 Shoucai Zhang, Shichao Liu, Congbo Yue, Yingjie Liu, Guixi Zheng, Yi Zhang
This study aims to identify the potential necroptosis related genes (NRGs)-associated miRNAs signature and explore the impact on the prognosis of stomach adenocarcinoma (STAD). Employing rigorous methodologies, we utilized univariate Cox, Lasso and multivariate Cox regression analyses to develop a prognostic signature. Kaplan-Meier (K-M) and ROC curves were applied to assess the prognostic value of
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Botulinum toxin type a blocks aquaporin 5 trafficking by decreasing synaptosomal-associated protein 23 in submandibular acinar cells Exp. Cell Res. (IF 3.7) Pub Date : 2024-02-01 Hui Xu, Huabing Ge, Zhigang Cai
The trafficking of aquaporin 5 (AQP5) is critical for salivary secretion. Synaptosomal-associated protein 23 (SNAP23) is an important regulator in the process of membrane fusion. However, the role of SNAP23 on AQP5 trafficking has not been explored. Botulinum toxin type A (BoNT/A) is a bacterial toxin that effectively treats sialorrhea. We previously reported that BoNT/A induced AQP5 redistribution
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Effect of sodium butyrate treatment at the basolateral membranes on the tight junction barrier function via a monocarboxylate transporter in goat mammary epithelial cells Exp. Cell Res. (IF 3.7) Pub Date : 2024-02-01 Yusaku Tsugami, Naoki Suzuki, Takahiro Nii, Naoki Isobe
In lactating mammary glands, tight junctions (TJs) prevent blood from mixing with milk and maintain epithelial cell polarity, which is important for milk production. This study aimed to investigate the effect of sodium acetate and sodium butyrate (SB) stimulation direction on the TJ barrier function, which is measured with regard to transepithelial electrical resistance and fluorescein flux, in goat
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Pterostilbene upregulates MICA/B via the PI3K/AKT signaling pathway to enhance the capability of natural killer cells to kill cervical cancer cells Exp. Cell Res. (IF 3.7) Pub Date : 2024-02-01 Zuoping Li, Jiaru Zhang, Shiwan You, Jing Zhang, Yuling Zhang, Zubair Akram, Shiguo Sun
Natural killer (NK) cells are triggered by the innate immune response in the tumor microenvironment. The extensive set of stimulating and inhibiting receptors mediates the target recognition of NK cells, and controls the strength of the effector reaction countering specific targeted cells. Yet, lacking major MHC (histocompatibility complex) MICA/B class I chain-related proteins on the membrane of tumor
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Lysine demethylase KDM3A alleviates hyperoxia-induced bronchopulmonary dysplasia in mice by promoting ETS1 expression Exp. Cell Res. (IF 3.7) Pub Date : 2024-01-28 Min Yang, Yanping Chen, Xueshan Huang, Fang Shen, Yanni Meng
Bronchopulmonary dysplasia (BPD) is the most common chronic lung disease among neonates, with increasing morbidity and mortality. This study aims to investigate the effect and mechanism of lysine demethylase 3A (KDM3A) on hyperoxia-induced BPD. Hyperoxia-induced BPD mouse and alveolar epithelial cell models were constructed. The effects of hyperoxia on lung development were evaluated by histological
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The m6A methyltransferase RBM15 affects tumor cell stemness and progression of cervical cancer by regulating the stability of lncRNA HEIH Exp. Cell Res. (IF 3.7) Pub Date : 2024-01-26 Yi Quan, Muchuan Zhou, Jinhong Li, Yihong Yang, Junliang Guo, Tian Tang, Ping Liu
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Characterization and identification of the insc-GAL4 driver that marks distinct cell types in the Drosophila midgut Exp. Cell Res. (IF 3.7) Pub Date : 2024-01-24 Akanksha Pandey, Jagat Kumar Roy
Drosophila geneticists frequently employ the binary GAL4-UAS system of conditional gene expression to direct expression of the desired transgene in tissues of interest. The inscuteable -GAL4 driver (insc-GAL4) expresses in the type 1 and type 2 neuroblasts of Drosophila larval brain, a frequent target tissue in many investigations. This GAL4 line additionally displayed its expression in the midgut
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RBM10 regulates the tumorigenic potential of human cancer cells by modulating PPM1B and YBX1 activities Exp. Cell Res. (IF 3.7) Pub Date : 2024-01-20 Yueyang Li, Di Wei, Zixiang Chen, Yukun Chen, Yuchun Deng, Mengge Li, Yongliang Zhao, Kaifeng Niu
RNA binding protein RBM10 participates in various RNA metabolism, and its decreased expression or loss of function by mutation has been identified in many human cancers. However, how its dysregulation contributes to human cancer pathogenesis remains to be determined. Here, we found that RBM10 expression was decreased in breast tumors, and breast cancer patients with low RBM10 expression presented poorer
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Thyroid cancer cell metabolism: A glance into cell culture system-based metabolomics approaches Exp. Cell Res. (IF 3.7) Pub Date : 2024-01-24 Raziyeh Abooshahab, Fatemeh Razavi, Fatemeh Ghorbani, Koroush Hooshmand, Maryam Zarkesh, Mehdi Hedayati
Thyroid cancer is the most common malignancy of the endocrine system and the seventh most prevalent cancer in women worldwide. It is a complex and diverse disease characterized by heterogeneity, underscoring the importance of understanding the underlying metabolic alterations within tumor cells. Metabolomics technologies offer a powerful toolset to explore and identify endogenous and exogenous biochemical
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Correction of Ito in human induced pluripotent stem Cell–derived cardiomyocyte carrying DPP6 mutation in early repolarization syndrome by CRISPR/Cas9 genome editing Exp. Cell Res. (IF 3.7) Pub Date : 2024-01-24 Yang Wu, Xiang-Jun Kong, Ying-Ying Ji, Jun Fan, Xu-Miao Chen, Cheng-Cheng Ji, Yun-Jiu Cheng, Su-Hua Wu
Early repolarization syndrome (ERS) is defined as occurring in patients with early repolarization pattern who have survived idiopathic ventricular fibrillation with clinical evaluation unrevealing for other explanations. The pathophysiologic basis of the ERS is currently uncertain. The objective of the present study was to examine the electrophysiological mechanism of ERS utilizing induced pluripotent
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HECW1 restrains cervical cancer cell growth by promoting DVL1 ubiquitination and downregulating the activation of Wnt/β-catenin signaling Exp. Cell Res. (IF 3.7) Pub Date : 2024-01-23 Zhen Xu, Yilin Guo, Lu Wang, Jinquan Cui
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CEBPA facilitates LOXL2 and LOXL3 transcription to promote BCL-2 stability and thus enhances the growth and metastasis of lung carcinoma cells in vitro Exp. Cell Res. (IF 3.7) Pub Date : 2024-01-17 Lihua Fan, Weijuan Jiang, Canjuan Chen, Hong Gao, Jiangwei Shi, Dewen Wang
Lung carcinoma (LC) is a complicated and highly heterogeneous disease with high morbidity and mortality. Both lysyl oxidase-like (LOXL) 2 and 3 act in cancer progression. This work endeavors to illustrate the influence of LOXL2/LOXL3 on LC progression and the underlying mechanisms. LOXL family genes and CCAAT enhancer binding protein A (CEBPA) were analyzed in the TCGA database for their expression
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Transcriptional mechanism of E2F1/TFAP2C/NRF1 in regulating KANK2 gene in nephrotic syndrome Exp. Cell Res. (IF 3.7) Pub Date : 2024-01-20 Yan Mao, Yan Zhou, Yan Chen, Rong Xu, Yi-Qing Wu, Wei-Wei Zhu, Xu-Fang Wang, Qian Wang, Chen-Xia Juan
The mortality rate linked with nephrotic syndrome (NS) is quite high. The renal tubular injury influences the response of NS patients to steroid treatment. KN motif and ankyrin repeat domains 2 (KANK2) regulates actin polymerization, which is required for renal tubular cells to maintain their function. In this study, we found that the levels of KANK2 in patients with NS were considerably lower than
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Preparation and in vitro evaluation of cell adhesion and long-term proliferation of stem cells cultured on silibinin co-embedded PLGA/Collagen electrospun composite nanofibers Exp. Cell Res. (IF 3.7) Pub Date : 2024-01-14 Mehdi Dadashpour, Shaylan Kalavi, Amirsasan Gorgzadeh, Rahim Nosrati, Akram Firouzi Amandi, Meysam Mohammadikhah, Mehdi Rezai Seghin Sara, Effat Alizadeh
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CLSPN actives Wnt/β-catenin signaling to facilitate glycolysis and cell proliferation in oral squamous cell carcinoma Exp. Cell Res. (IF 3.7) Pub Date : 2024-01-17 Zeyu Hou, Chenzhou Wu, Jinru Tang, Shaohua Liu, Longjiang Li
Objective Oral squamous cell carcinoma (OSCC) is a common malignancy with a poor prognosis. This study aimed to determine the influence and underlying mechanisms of CLSPN on OSCC. Methods CLSPN expression was tested using quantitative real-time polymerase chain reaction, immunohistochemistry, and western blotting. Flow cytometry, cell counting kit, and colony formation assays were performed to determine
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Hic-5 regulates extracellular matrix-associated gene expression and cytokine secretion in cancer associated fibroblasts Exp. Cell Res. (IF 3.7) Pub Date : 2024-01-17 Weiyi Xu, Gregory J. Goreczny, Ian Forsythe, Grant Brennan, Theresa Stowell, Katia Brock, Benjamin Capella, Christopher E. Turner
The focal adhesion protein, Hic-5 plays a key role in promoting extracellular matrix deposition and remodeling by cancer associated fibroblasts within the tumor stroma to promote breast tumor cell invasion. However, whether stromal matrix gene expression is regulated by Hic-5 is still unknown. Utilizing a constitutive Hic-5 knockout, Mouse Mammary Tumor Virus-Polyoma Middle T-Antigen spontaneous breast
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Direct differentiation of rat skin fibroblasts into cardiomyocytes Exp. Cell Res. (IF 3.7) Pub Date : 2024-01-17 Waqas Ahmad, Bilal Saleh, Rida-e-Maria Qazi, Rabbia Muneer, Irfan Khan, Mohsin Khan, Asmat Salim
Myocardial infarction (MI) is one of the major cardiovascular diseases caused by diminished supply of nutrients and oxygen to the heart due to obstruction of the coronary artery. Different treatment options are available for cardiac diseases, however, they do not completely repair the damage. Therefore, reprogramming terminally differentiated fibroblasts using transcription factors is a promising strategy
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Corrigendum to “Downregulation of CYRI-B promotes migration, invasion and EMT by activating the Rac1-STAT3 pathway in gastric cancer” [423(2023)/113453] Exp. Cell Res. (IF 3.7) Pub Date : 2024-01-12 Guangyao Liu, Yujian Xia, Huijin Wang, Xinghan Jin, Songyao Chen, Wei Chen, Changhua Zhang, Yulong He
Abstract not available
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MiR-92a-2-5p suppresses esophageal squamous cell carcinoma cell proliferation and invasion by targeting PRDX2 Exp. Cell Res. (IF 3.7) Pub Date : 2024-01-09 Xiang Zhan, Jixian Li, Renya Zeng, Lingli Lei, Alei Feng, Zhe Yang
MicroRNAs (miRNAs) can function as negative regulators of gene expression by binding to the 3′-untranslated region (3′-UTR) of target genes. The aberrant expression of miRNAs in neoplasm is extensively associated with tumorigenesis and cancer progression, including esophageal squamous cell carcinoma (ESCC). Our previous investigation has identified the oncogenic roles of Peroxiredoxin2 (PRDX2) in ESCC
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CAMSAP3, a microtubule orientation regulator, plays a vital role in manifesting differentiation-dependent characteristics in keratinocytes Exp. Cell Res. (IF 3.7) Pub Date : 2024-01-07 Mako Kobori, Junya Abe, Reika Saito, Yohei Hirai
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MAPRE3 as an epigenetic target of EZH2 restricts ovarian cancer proliferation in vitro and in vivo Exp. Cell Res. (IF 3.7) Pub Date : 2024-01-08 Yun Zhang, Fanglin Liu, Jindan Zheng, Keping Jiang, Hao Ai, Lili Liu, Dong Mao
Ovarian cancer (OC) is a lethal gynecologic cancer and the common cause of death within women worldwide. The polycomb group protein enhancer of zeste homolog 2 (EZH2) is a histone methyltransferase highly expressed in various tumors, including OC. However, the mechanistic basis of EZH2 oncogenic activity in OC remain incompletely understood. Bioinformatics analysis showed that the expression of MAPRE3
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Glycyrrhizin attenuates myocardial ischemia reperfusion injury by suppressing Inflammation, oxidative stress, and ferroptosis via the HMGB1-TLR4-GPX4 pathway Exp. Cell Res. (IF 3.7) Pub Date : 2024-01-02 Kaiyi Zhu, Rong Fan, Yuchen Cao, Wei Yang, Zhe Zhang, Qiang Zhou, Jie Ren, Xiushan Shi, Yuping Gao, Xiang Guo
Ferroptosis, a form of regulated cell death process, play an important role in myocardial ischemia‒reperfusion (I/R) injury. Glycyrrhizin (GL), a natural glycoconjugate triterpene, has the property to improve growth rate, immune regulation, antioxidant, anti-inflammatory. However, whether GL can attenuate myocardial I/R injury by modulating ferroptosis or other mechanisms are still unclear. In this
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Caveolae-mediated endocytosis of extracellular QSOX1b modulates the migration of fibroblasts Exp. Cell Res. (IF 3.7) Pub Date : 2024-01-02 Pierina A. Martinez, Silvio M. Zanata, Lia S. Nakao