The 2017 Nobel Prize in Chemistry was awarded to Jacques Dubochet, Joachim Frank, and Richard Henderson for “developing cryo-electron microscopy (cryo-EM) for the high-resolution structure determination of biomolecules in solution.” This feature article summarizes some of the major achievements leading to the development of cryo-EM and recent technological breakthroughs that have transformed the method into a mainstream tool for structure determination.

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2017年诺贝尔化学奖：cryo-EM走向成熟

Jacques Dubochet，Joachim Frank和Richard Henderson因“开发用于溶液中生物分子的高分辨率结构测定的低温电子显微镜（cryo-EM）”而获得2017年诺贝尔化学奖。这篇专题文章总结了导致cryo-EM发展的一些主要成就以及最近的技术突破，这些成就已将方法转变为结构确定的主流工具。