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Establishment of pressurized-liquid extraction by response surface methodology approach coupled to HPLC-DAD-TOF-MS for the determination of phenolic compounds of myrtle leaves
Analytical and Bioanalytical Chemistry ( IF 4.3 ) Pub Date : 2018-02-09 , DOI: 10.1007/s00216-018-0914-0
Elixabet Díaz-de-Cerio , David Arráez-Román , Antonio Segura-Carretero , Pasquale Ferranti , Rosario Nicoletti , Giuseppe Mirko Perrotta , Ana María Gómez-Caravaca

Myrtus communis L. (myrtle) is native to the Mediterranean region and Western Asia. Its leaves have demonstrated its potential effect towards different bioactivities like anti-diabetic, anti-diarrheic, anti-ulcer, anti-cancer, among others. These activities have been associated with its phenolic content. In this sense, the aim of this work has been to develop a new pressurized-liquid extraction procedure (PLE), by using a response surface methodology (RSM), to evaluate the phenolic composition from myrtle leaves by HPLC-DAD-TOF-MS. Previously, different solvents such as methanol, ethanol, and acetone/water mixtures were tested by using ultrasound-assisted extraction (UAE) in order to select the most suitable one. Subsequently, a Box-Behnken design (BBD) was performed according to the effect of ethanol/water ratio (50, 75, and 100% (v/v)), temperature (50, 125, and 200 °C), and extraction time (5, 18, and 30 min). The optimal conditions achieved with the established method were 71% ethanol/water, 137 °C, and 19 min. The analysis of the obtained extracts by HPLC-DAD-TOF-MS allowed the characterization of 15 new compounds in myrtle leaves. Finally, high amounts of gallic and ellagic acid were found in the optimized PLE extracts (3.31 ± 0.03 and 3.88 ± 0.09 mg/g leaf dry weight (d.w.), respectively), and PLE reported greater recovery of total phenolic compounds than UAE (30 ± 1 and 22.4 ± 0.6 mg/g leaf d.w., respectively).



中文翻译:

响应面分析法与HPLC-DAD-TOF-MS联用建立加压液体萃取法测定桃金娘叶中的酚类化合物

桃金娘L.(桃金娘)原产于地中海地区和西亚。它的叶子已经显示出其对不同生物活性的潜在作用,例如抗糖尿病,抗腹泻,抗溃疡,抗癌等。这些活性与其酚含量有关。从这个意义上讲,这项工作的目的是通过使用响应表面方法(RSM)开发一种新的加压液体萃取程序(PLE),以通过HPLC-DAD-TOF-MS评价桃金娘叶中的酚类成分。以前,通过使用超声辅助萃取(UAE)对不同的溶剂(例如甲醇,乙醇和丙酮/水混合物)进行了测试,以选择最合适的溶剂。随后,根据乙醇/水比例(50%,75%和100%(v/ v)),温度(50、125和200°C)和萃取时间(5、18和30分钟)。用已建立的方法获得的最佳条件是71%的乙醇/水,137°C和19分钟。通过HPLC-DAD-TOF-MS对获得的提取物进行分析,可以鉴定桃金娘叶片中的15种新化合物。最后,在优化的PLE提取物中发现了大量的没食子酸和鞣花酸(分别为3.31±0.03和3.88±0.09 mg / g叶片干重(dw)),并且PLE报告的总酚类化合物回收率高于阿联酋(30)叶dw分别为±1和22.4±0.6 mg / g dw)。

更新日期:2018-02-09
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