Research into the regulation of gene expression underwent a shift from focusing on DNA-binding proteins as key transcriptional regulators to RNA-binding proteins (RBPs) that come into play once transcription has been initiated. RBPs orchestrate all RNA-processing steps in the cell. To obtain a global view of in vivo targets, the RNA complement associated with particular RBPs is determined via immunoprecipitation of the RBP and subsequent identification of bound RNAs via RNA-seq. Here, we describe technical advances in identifying RBP in vivo targets and their binding motifs. We provide an up-to-date view of targets of nucleocytoplasmic RBPs collected in arabidopsis. We also discuss current experimental limitations and provide an outlook on how the approaches may advance our understanding of post-transcriptional networks.

对基因表达调控的研究经历了从专注于作为主要转录调节因子的DNA结合蛋白到转录开始就起作用的RNA结合蛋白（RBP）的转变。RBP协调细胞中所有RNA加工步骤。为了获得体内靶标的全局视图，与特定RBP相关的RNA补体是通过RBP的免疫沉淀和随后通过RNA-seq鉴定结合的RNA来确定的。在这里，我们描述了体内识别RBP的技术进展靶及其结合基序。我们提供了在拟南芥中收集的核细胞质RBP靶标的最新视图。我们还讨论了当前的实验局限性，并就这些方法如何增进我们对转录后网络的理解提供了展望。