A synthetic topology for everted viruses is reported. The topology is a single-stranded virion DNA assembled into a hollow cube with exterior decorated with HIV-Tat transduction domains. The cube incorporates a pH-responsive lid allowing for the controlled encapsulation of functional proteins and their transfer and release into live cells. Unlike viruses, which are protein shells with a [3,5]-fold rotational symmetry that encase nucleic acids, these cubes are [3, 4]-fold DNA boxes encapsulating proteins. Like viruses, such everted DNA-built viruses are monodisperse nanoscale assemblies that infect human cells with a specialist cargo. The design offers a bespoke bottom-up platform for engineering nonpolyhedral, nonprotein synthetic viruses.

中文翻译：

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DNA折纸里里外外病毒

报告了外翻病毒的合成拓扑。拓扑结构是单链病毒粒子DNA，组装成空心立方体，外部装饰有HIV-Tat转导域。多维数据集包含一个pH响应盖，可对功能蛋白进行可控封装，并将其转移并释放到活细胞中。与病毒不同，病毒是包裹核酸的具有[3,5]倍旋转对称性的蛋白质壳，这些立方体是包裹蛋白质的[3，4]倍DNA盒。像病毒一样，这种外翻的DNA构建病毒是单分散的纳米级组件，会用专门的货物感染人类细胞。该设计为工程非多面体，非蛋白质合成病毒提供了定制的自底向上平台。