The present work introduces composite luminescent nanoparticles (Ag⁰-Tb³⁺-SNs), where ultra-small nanosilver (4 ± 2 nm) is deposited onto amino-modified silica nanoparticles (35±6 nm) doped by green luminescent Tb(III) complexes. Ag⁰-Tb³⁺-SNs are able to image cancer (Hep-2) cells in confocal microscopy measurements due to efficient cell internalization, which is confirmed by TEM images of the Hep-2 cells exposed by Ag⁰-Tb³⁺-SNs. Comparative analysis of the cytotoxicity of normal fibroblasts (DK-4) and cancer cells (Hep-2) incubated with various concentrations of Ag⁰-Tb³⁺-SNs revealed the concentration range where the toxic effect on the cancer cells is significant, while it is insignificant towards the nonmalignant fibroblasts cells. The obtained results reveal Ag⁰-Tb³⁺-SNs as good cellular contrast agent able to induce the cancer cells death, which makes them promising theranostic in cancer diagnostics and therapy.

本工作介绍了复合发光纳米颗粒（Ag ⁰ -Tb ³⁺ -SNs），其中将超小型纳米银（4±2 nm）沉积在掺杂有绿色发光Tb（III）的氨基改性二氧化硅纳米颗粒（35±6 nm）上）复合体。由于有效的细胞内在化，Ag ⁰ -Tb ³⁺ -SNs能够在共聚焦显微镜测量中对癌细胞（Hep-2）成像，这通过Ag ⁰ -Tb ³⁺ -暴露的Hep-2细胞的TEM图像得以证实。SN。正常成纤维细胞（DK-4）和癌细胞（Hep-2）与不同浓度的Ag ⁰ -Tb ³⁺孵育后的细胞毒性比较分析-SNs显示了浓度范围，其中对癌细胞的毒性作用是显着的，而对非恶性成纤维细胞则是微不足道的。获得的结果表明，Ag ⁰ -Tb ³⁺ -SNs是能够诱导癌细胞死亡的良好细胞造影剂，这使得它们有望在癌症诊断和治疗中发挥治疗作用。