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Hyperpolarization by N-(3-oxododecanoyl)-l-homoserine-lactone, a quorum sensing molecule, in rat thymic lymphocytes
Chemico-Biological Interactions ( IF 5.1 ) Pub Date : 2018-02-08 , DOI: 10.1016/j.cbi.2018.02.008
Yumiko Nishimura-Danjobara , Keisuke Oyama , Kumio Yokoigawa , Yasuo Oyama

To study the adverse effects of N-(3-oxododecanoyl)-l-homoserine-lactone (ODHL), a quorum sensing molecule, on mammalian host cells, its effect on membrane potential was examined in rat thymic lymphocytes using flow cytometric techniques with a voltage-sensitive fluorescent probe. As 3–300 μM ODHL elicited hyperpolarization, it is likely that it increases membrane K+ permeability because hyperpolarization is directly linked to changing K+ gradient across membranes, but not Na+ and Cl gradients. ODHL did not increase intracellular Ca2+ concentration. ODHL also produced a response in the presence of an intracellular Zn2+ chelator. Thus, it is unlikely that intracellular Ca2+ and Zn2+ are attributed to the response. Quinine, a non-specific K+ channel blocker, greatly reduced hyperpolarization. However, because charybdotoxin, tetraethylammonium chloride, 4-aminopyridine, and glibenclamide did not affect it, it is pharmacologically hypothesized that Ca2+-activated K+ channels, voltage-gated K+ channels, and ATP-sensitive K+ channels are not involved in ODHL-induced hyperpolarization. Although the K+ channels responsible for ODHL-induced hyperpolarization have not been identified, it is suggested that ODHL can elicit hyperpolarization in mammalian host cells, disturbing cellular functions.



中文翻译:

N-(3-氧十二烷酰基)-1-高丝氨酸-内酯,一种群体感应分子,在大鼠胸腺淋巴细胞中超极化

为了研究群体感应分子N-(3-氧十二烷酰基)-1-高丝氨酸-内酯(ODHL)对哺乳动物宿主细胞的不利影响,使用流式细胞仪检测了其对大鼠胸腺淋巴细胞膜电位的影响。电压敏感型荧光探针。如3-300μMODHL引起超极化,则很可能会增加膜K +渗透性,因为超极化直接连接到切换K +跨膜梯度,而不是钠+和Cl -梯度。ODHL不会增加细胞内Ca 2+的浓度。在细胞内Zn 2+存在的情况下,ODHL也会产生反应螯合剂。因此,不可能将细胞内Ca 2+和Zn 2+归因于该响应。奎宁是一种非特异性的K +通道阻滞剂,可大大减少超极化现象。但是,由于炭疽毒素,氯化四乙铵,4-氨基吡啶和格列本脲没有影响,因此从药理学上讲,不涉及Ca 2+激活的K +通道,电压门控的K +通道和ATP敏感的K +通道。在ODHL引起的超极化。虽然K + 尚未确定负责ODHL诱导的超极化的通道,这表明ODHL可以在哺乳动物宿主细胞中引起超极化,从而干扰细胞功能。

更新日期:2018-02-08
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