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Tracking fast cellular membrane dynamics with sub-nm accuracy in the normal direction†
Nanoscale ( IF 6.7 ) Pub Date : 2018-02-07 00:00:00 , DOI: 10.1039/c7nr09483c
Hui Yu 1 , Yuting Yang , Yunze Yang , Fenni Zhang , Shaopeng Wang , Nongjian Tao
Affiliation  

Cellular membranes are important biomaterials with highly dynamic structures. Membrane dynamics plays an important role in numerous cellular processes, but precise tracking it is challenging due to the lack of tools with a highly sensitive and fast detection capability. Here we demonstrate a broad bandwidth optical imaging technique to measure cellular membrane displacements in the normal direction at sub-nm level detection limits and 20 μs temporal resolution (1 Hz–50 kHz). This capability allows us to study the intrinsic cellular membrane dynamics over a broad temporal and spatial spectrum. We measured the nanometer-scale stochastic fluctuations of the plasma membrane of HEK-293 cells, and found them to be highly dependent on the cytoskeletal structure of the cells. By analyzing the fluctuations, we further determine the mechanical properties of the cellular membranes. We anticipate that the method will contribute to the understanding of the basic cellular processes, and applications, such as mechanical phenotyping of cells at the single-cell level.

中文翻译:

在法向方向上以亚纳米精度跟踪快速细胞膜动力学†

细胞膜是具有高度动态结构的重要生物材料。膜动力学在许多细胞过程中发挥着重要作用,但由于缺乏具有高灵敏度和快速检测能力的工具,对其进行精确跟踪具有挑战性。在这里,我们展示了一种宽带宽光学成像技术,可在亚纳米级检测限和 20 μs 时间分辨率 (1 Hz–50 kHz) 下测量法线方向的细胞膜位移。这种能力使我们能够在广泛的时间和空间范围内研究内在的细胞膜动力学。我们测量了 HEK-293 细胞质膜的纳米级随机波动,发现它们高度依赖于细胞的细胞骨架结构。通过分析波动,我们进一步确定细胞膜的机械性能。我们预计该方法将有助于理解基本的细胞过程和应用,例如单细胞水平的细胞机械表型。
更新日期:2018-02-07
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