We report a chemically tunable electronic sensor for quantitation of gluten based on a floating-gate transistor (FGT) architecture. The FGTs are fabricated in parallel and each one is functionalized with a different chemical moiety designed to preferentially bind a specific grain source of gluten. The resulting set of FGT sensors can detect both wheat and barley gluten below the gluten-free limit of 20 ppm (w/w) while providing a source-dependent signature for improved accuracy. This label-free transduction method does not require any secondary binding events, resulting in a ca. 45 min reduction in analysis time relative to state-of-the-art ELISA kits with a simple and easily implemented workflow.

中文翻译：

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多个浮栅晶体管生物传感器对谷物中谷蛋白的检测和来源

我们报告基于浮栅晶体管（FGT）架构的面筋定量的化学可调电子传感器。FGT是平行制造的，每个都由不同的化学部分官能化，这些化学部分旨在优先结合特定的谷蛋白谷物来源。所得的FGT传感器组可以检测低于20 ppm（w / w）无麸质极限的小麦和大麦麸质，同时提供源相关的特征以提高准确性。这种无标记的转导方法不需要任何二次结合事件，从而导致大约 与最先进的ELISA试剂盒相比，其分析时间缩短了45分钟，且工作流程简单易行。