Reactive pentafluorophenyl acrylate (PFPA) polymer brushes grafted on silica particles were prepared using surface-initiated reversible addition and fragmentation chain transfer polymerization. The polymer brush was successfully immobilized with antibody, then used for protein separation. The immunoprecipitated proteins showed successful enrichment of target protein, with reduced nonspecific background and less contamination from eluted antibodies. To further improve protein recovery, the hydrophobic poly(PFPA) brush was modified with hydrophilic poly(ethylene glycol) (PEG). The partially PEG-substituted poly(PFPA) brush showed better dispersion in aqueous solution, leading to improved antibody immobilization efficiency. By optimizing both the brush molecular weight and the degree of PEG substitution, an optimal balance between surface hydrophilicity and number of available PFP units was found, leading to efficient target protein purification. This study shows that poly(PFPA) platform offers a versatile approach to prepare biomolecule-activated surfaces with tunable surface property, which has potential applications in protein separation and other areas.

中文翻译：

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胺反应性聚五氟苯基丙烯酸酯刷平台，用于更清洁的蛋白质纯化

使用表面引发的可逆加成和断裂链转移聚合反应制备接枝在二氧化硅颗粒上的反应性丙烯酸五氟苯基酯（PFPA）聚合物刷。聚合物刷成功地固定了抗体，然后用于蛋白质分离。免疫沉淀的蛋白质显示出目标蛋白质的成功富集，非特异性背景减少，洗脱抗体的污染减少。为了进一步提高蛋白质回收率，疏水性聚（PFPA）刷子用亲水性聚（乙二醇）（PEG）改性。部分PEG取代的聚（PFPA）刷子在水溶液中显示出更好的分散性，从而提高了抗体的固定效率。通过优化刷子的分子量和PEG的取代程度，发现表面亲水性和可用PFP单元数之间的最佳平衡，从而实现了有效的靶蛋白纯化。这项研究表明，poly（PFPA）平台提供了一种通用的方法来制备具有可调表面特性的生物分子活化的表面，这在蛋白质分离和其他领域中具有潜在的应用。