In vivo monitoring of dopamine (DA) in cerebral nervous system with no traumatic brain injury is still a challenging work for reliably evaluating the role that DA plays in physical and cognitive functionality. In this work, to reduce the traumatic injury on the brain tissue, we develop a glass‐sealed Au nanoelectrode (GSNE) with nanoscale physical dimention for cerebral DA detection. First, an Au microwire was sealed into the pre‐thinned silica capillary. Then, the obtained Au/capillary assembly was pulled into two tips followed by polishing away the excess glass to expose the Au nanotip. The morphology of GSNE was characterized by scanning electron microscopy and transmission electron microscopy. The results showed that this nanoelectrode can be quickly fabricated with high reproducibility. By further electrodepositing cluster‐like gold nanostructure on GSNE and modifying Nafion, amperometric detection of DA with high sensitivity and good selectivity were achieved on the Nafion‐modified Au/GSNE. Moreover, the Nafion‐modified Au/GSNE possessed advantages of easy tissue insertion and minimum tissue disturbance. Thus, the developed electrochemical nanosensor was successfully used for amperometrically monitoring DA level in the striatum of anesthetic rats.

中文翻译：

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用于大鼠脑中多巴胺体内检测的玻璃密封金纳米电极的开发

体内监测脑神经系统中的多巴胺（DA）且没有创伤性脑损伤，对于可靠地评估DA在身体和认知功能中的作用仍然是一项艰巨的工作。在这项工作中，为了减少对脑组织的创伤，我们开发了具有纳米级物理尺寸的玻璃密封金纳米电极（GSNE），用于脑DA检测。首先，将一条金纳米线密封到预稀释的硅胶毛细管中。然后，将获得的Au /毛细管组件拉入两个尖端，然后抛光掉多余的玻璃以暴露出Au纳米尖端。GSNE的形态通过扫描电子显微镜和透射电子显微镜表征。结果表明，该纳米电极可以以高再现性快速制备。通过在GSNE上进一步电沉积簇状金纳米结构并修饰Nafion，可以在Nafion修饰的Au / GSNE上以高灵敏度和良好的选择性对DA进行安培检测。此外，Nafion修饰的Au / GSNE具有易于组织插入和最小化组织干扰的优点。因此，开发的电化学纳米传感器已成功用于安培法监测麻醉大鼠纹状体中DA水平。