In this contribution, we want to review recent advances and innovations in laser sources and detection concepts for coherent Raman scattering microscopy with special emphasis on hyperspectral and multiplex imaging methods for biomedical applications. Groundbreaking advances have been made to increase the chemical sensitivity while maintaining video-rate imaging speeds. Excitation and detection schemes have been further improved to enhance the image contrast as well as to increase the spectral coverage and speed of multi-spectral data acquisition along with the ongoing quest to develop both compact and robust but also powerful and rapidly tunable laser sources suitable for integration into biomedical equipment.

在此贡献中，我们要回顾相干拉曼散射显微镜在激光源和检测概念方面的最新进展和创新，特别是针对生物医学应用的高光谱和多重成像方法。在保持视频速率成像速度的同时提高化学敏感性方面取得了突破性进展。激励和检测方案已得到进一步改进，以增强图像对比度，并提高多光谱数据采集的光谱覆盖范围和速度，并不断寻求开发适用于紧凑，坚固，功能强大且快速可调的激光源，集成到生物医学设备中。