A direct laser bioprinting (DLBP) system is introduced in this work. The DLBP system applies visible‐laser‐induced photo‐crosslinking at a wavelength of 405 nm using the photoinitiator VA‐086. It is shown that such a system can fabricate vertical structures with fine features (less than 50 µm) and high cell viability (greater than 95%). Experimental characterizations and theoretical simulations are presented, and good agreement is seen between the experiments and theory. The DLBP system is applied to the fabrication of (1) cell‐laden hydrogel microgrids, (2) hydrogel microwells, as well as a test of (3) cell encapsulation, and (4) cell seeding. The DLBP system is found to be a promising tool for bioprinting.

中文翻译：

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一种新颖的，分辨率良好的直接激光生物打印系统，用于快速细胞包封和微孔加工

在这项工作中介绍了直接激光生物打印（DLBP）系统。DLBP系统使用光引发剂VA-086在405 nm的波长上应用可见光诱导的光交联。结果表明，这种系统可以制造出具有精细特征（小于50 µm）和高细胞生存力（大于95％）的垂直结构。给出了实验表征和理论模拟，并在实验和理论之间看到了很好的一致性。DLBP系统可用于制造（1）装有细胞的水凝胶微格栅，（2）水凝胶微孔以及（3）细胞封装和（4）细胞接种的测试。发现DLBP系统是用于生物打印的有前途的工具。