Aucubin, an iridoid glycoside found in several plants, such as Eucommia ulmoide and Rehmannia, has various pharmacological effects. Bone formation is a complex process in which osteoblast differentiation plays an important role. This study aimed to investigate the promotion effects of aucubin on osteoblast differentiation in MG63 cells, a human osteoblast-like cell line. Aucubin not only improved osteoblast differentiation, as shown by enhanced ALP (alkaline phosphatase) concentration and mineralization in cells, but increased the expression of various cytokines, including collagen I, osteocalcin, osteopontin, integrin β1, and Osterix. Aucubin strongly enhanced the levels of BMP2 (bone morphogenetic proteins-2) in MG63 cells, which play a central role during osteoblast differentiation. Further data show that aucubin exposure after 1 day, 7 days, and 14 days enhanced the expression of Smad1, 5, and 8, and the phosphoresced levels of MAPKs (mitogen-activated protein kinases) family Erk (extracellular signal–regulated kinases), JNK (c-Jun-NH2-terminal kinases), P38, and Akt (serine/threonine protein kinase)/mTOR (mammalian target of rapamycin)/p70s6k in MG63 cells. This study shows the improved effects of aucubin on osteoblast differentiation in MG63 cells, related to the signaling of BMP2-mediated Smads (drosophila mothers against decapentaplegic proteins), MAPKs, and Akt/mTOR/p70S6K. This study indicates the potential of aucubin for osteoporosis treatment.

蜂胶，一种杜鹃花苷，存在于多种植物中，例如杜仲和熟地，具有多种药理作用。骨形成是一个复杂的过程，其中成骨细胞的分化起着重要的作用。这项研究的目的是研究aucubin对人成骨样细胞系MG63细胞中成骨细胞分化的促进作用。Aucubin不仅提高了ALP（碱性磷酸酶）的浓度和矿化作用，从而改善了成骨细胞的分化，而且增加了包括胶原蛋白I，骨钙素，骨桥蛋白，整联蛋白β1和Osterix在内的各种细胞因子的表达。Aucubin强烈增强了MG63细胞中BMP2（骨形态发生蛋白2）的水平，这在成骨细胞分化过程中起着核心作用。进一步的数据显示，在1天，7天和14天后暴露于洋红霉素会增强Smad1、5和8的表达。以及EAPK（细胞外信号调节激酶），JNK（c-Jun-NH2-末端激酶），P38和Akt（丝氨酸/苏氨酸蛋白激酶）/ mTOR（哺乳动物）MAPKs（丝裂原活化蛋白激酶）家族的磷酸化水平MG63细胞中的雷帕霉素靶标）/ p70s6k。这项研究表明，aucubin对MG63细胞中成骨细胞分化的改善作用，与BMP2介导的Smads（果蝇母亲抵抗十足功能蛋白），MAPKs和Akt / mTOR / p70S6K的信号传导有关。这项研究表明了洋红素治疗骨质疏松症的潜力。这项研究表明，aucubin对MG63细胞中成骨细胞分化的改善作用，与BMP2介导的Smads（果蝇母亲抵抗十足功能蛋白），MAPKs和Akt / mTOR / p70S6K的信号传导有关。这项研究表明了洋红素治疗骨质疏松症的潜力。这项研究表明，aucubin对MG63细胞中成骨细胞分化的改善作用，与BMP2介导的Smads（果蝇母亲抵抗十足功能蛋白），MAPKs和Akt / mTOR / p70S6K的信号传导有关。这项研究表明了洋红素治疗骨质疏松症的潜力。