Objective

Early responses of human pulp to Prime&Bond/phosphoric acid, Clearfil SE Bond, Clearfil S3 Bond and Dycal were investigated ex vivo.

Materials and methods

The three adhesives, Dycal or buffer (DPBS) were applied directly onto the pulp of human teeth slices that were placed in culture for 4 days. Cell viability was monitored by the MTT assay during the culture period. After 4 days, tissue integrity was examined by hematoxylin–eosin staining. Vimentin levels were assessed by Western blotting. TUNEL assay was applied for apoptotic cell detection at specific pulp areas.

Results

Profound reduction of cell viability and tissue integrity was observed in adhesive-treated groups, while the impact of Dycal was found to be less harmful. Extended apoptosis was caused mostly by the Clearfil SE and Prime&Bond. All adhesives reduced Vimentin levels.

Significance

The study provides evidence that early pulp responses to direct capping with different adhesive systems or calcium hydroxide may vary significantly and underline the need for further studies in relevant ex vivo systems.

中文翻译：

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人类牙髓对树脂粘合剂系统和氢氧化钙直接封盖的早期反应

客观的

离体研究了人类牙髓对Prime＆Bond /磷酸，Clearfil SE键，Clearfil S3键和Dycal的早期反应。

材料和方法

Dycal或缓冲液（DPBS）这三种粘合剂直接涂在人类牙齿切片的牙髓上，并在培养物中放置4天。在培养期间通过MTT测定法监测细胞活力。4天后，通过苏木精-伊红染色检查组织完整性。波形蛋白水平通过蛋白质印迹法评估。TUNEL测定法用于特定牙髓区域的凋亡细胞检测。

结果

在粘合剂处理组中观察到细胞活力和组织完整性的明显降低，而发现Dycal的影响危害较小。延长的细胞凋亡主要是由Clearfil SE和Prime＆Bond引起的。所有胶粘剂均降低波形蛋白水平。

意义

该研究提供的证据表明，早期牙髓对使用不同的粘合剂系统或氢氧化钙直接封盖的反应可能会发生显着变化，并强调需要在相关的离体系统中进行进一步研究。