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In vivo therapeutic applications of cell spheroids
Biotechnology Advances ( IF 16.0 ) Pub Date : 2018-02-03 , DOI: 10.1016/j.biotechadv.2018.02.003
Chin Siang Ong , Xun Zhou , Jingnan Han , Chen Yu Huang , Andrew Nashed , Shipra Khatri , Gunnar Mattson , Takuma Fukunishi , Huaitao Zhang , Narutoshi Hibino

Spheroids are increasingly being employed to answer a wide range of clinical and biomedical inquiries ranging from pharmacology to disease pathophysiology, with the ultimate goal of using spheroids for tissue engineering and regeneration. When compared to traditional two-dimensional cell culture, spheroids have the advantage of better replicating the 3D extracellular microenvironment and its associated growth factors and signaling cascades. As knowledge about the preparation and maintenance of spheroids has improved, there has been a plethora of translational experiments investigating in vivo implantation of spheroids into various animal models studying tissue regeneration.

We review methods for spheroid delivery and how they have been utilized in tissue engineering experiments. We break down efforts in this field by organ systems, discussing applications of spheroids to various animal models of disease processes and their potential clinical implications. These breakthroughs have been made possible by advancements in spheroid formation, in vivo delivery and assessment. There is unexplored potential and room for further research and development in spheroid-based tissue engineering approaches. Regenerative medicine and other clinical applications ensure this exciting area of research remains relevant for patient care.



中文翻译:

细胞球的体内治疗应用

越来越多地使用球体来回答从药理学到疾病病理生理学的各种临床和生物医学查询,其最终目标是将球体用于组织工程和再生。与传统的二维细胞培养相比,类球体具有更好地复制3D细胞外微环境及其相关生长因子和信号级联的优势。随着关于球体的制备和维持的知识的提高,已经进行了许多翻译实验,以体内研究将球体植入各种研究组织再生的动物模型中。

我们回顾了球体递送的方法,以及它们如何在组织工程实验中得到利用。我们通过器官系统细分了该领域的工作,讨论了球体在疾病过程的各种动物模型中的应用及其潜在的临床意义。通过突破球体形成,体内递送和评估,这些突破成为可能。基于球体的组织工程方法尚有未开发的潜力和进一步研究和发展的空间。再生医学和其他临床应用确保了这一令人振奋的研究领域仍然与患者护理相关。

更新日期:2018-02-03
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