In this work we report the synthesis of a stable composite with excellent electrical properties, on the surface of a biosensor. Conductive polymers offer both high electrical conductivity and mechanical strength. Many reports have focused on synthesizing conductive polymers with the aid of high-cost enzymes. In the current work we introduce a novel electrochemical, one-step, facile and cost effective procedure for synthesizing poly (catechol), without using expensive enzymes. The poly (catechol) conductivity was enhanced by modification with graphene sheets and biosynthesized gold nanoparticles. Four different robust methods, DPV, EIS, CV and chronoamperometry, were used to monitor the biosensor modifications. The peak currents of the catechol (an electroactive probe) were linearly related to the logarithm of the concentrations of target DNA in the range 100.0 μM to 10.0 pM, with a detection limit of 1.0 pM for the DNA strand. The current work investigates a new, stable composite consisting of conductive polymers and nanoparticles, which was applied to the detection of acute lymphoblastic leukemia.

在这项工作中，我们报告了在生物传感器表面上合成具有优良电性能的稳定复合材料的过程。导电聚合物既提供高电导率又提供机械强度。许多报道集中于借助高成本的酶来合成导电聚合物。在当前的工作中，我们介绍了一种新颖的，一步法，简便且经济高效的合成聚邻苯二酚的方法，而无需使用昂贵的酶。通过用石墨烯片和生物合成的金纳米粒子进行改性，可以提高聚（邻苯二酚）的电导率。DPV，EIS，CV和计时电流法这四种不同的鲁棒方法用于监视生物传感器的修改。儿茶酚（电活性探针）的峰值电流与目标DNA浓度在100.0μM到10.0 pM之间的对数线性相关，对于DNA链的检测极限为1.0 pM。目前的工作是研究一种由导电聚合物和纳米颗粒组成的新型，稳定的复合材料，该复合材料已用于检测急性淋巴细胞白血病。