A protein delivery tool based on bacterial type III secretion system (T3SS) has been broadly applied in biomedical researches. In this review, we summarize various applications of the T3SS-mediate protein delivery which enables translocation of proteins directly into mammalian cells without protein purification. Some of the remarkable advancements include delivery of antigens for therapeutic vaccines, nucleases for genome editing, transcription factors for cellular reprogramming and stem cells differentiation, and signaling molecules for post-translational proteomics studies. With continued improvement of the T3SS-mediated protein delivery tools, even wider application of the technology is anticipated.

基于细菌III型分泌系统（T3SS）的蛋白质递送工具已广泛应用于生物医学研究。在这篇综述中，我们总结了T3SS介导的蛋白质递送的各种应用，该应用使蛋白质无需蛋白质纯化即可直接转运到哺乳动物细胞中。一些显着的进步包括用于治疗性疫苗的抗原递送，用于基因组编辑的核酸酶，用于细胞重编程和干细胞分化的转录因子，以及用于翻译后蛋白质组学研究的信号分子。随着T3SS介导的蛋白质递送工具的不断改进，预计该技术将得到更广泛的应用。