Don't FRET! We describe a method that enables fast site‐specific fluorescent labelling of human proteins produced in their endogenous cellular environment with external chemical groups such as fluorescent dyes and an experimental strategy to make the labelled proteins amenable for single‐molecule FRET analysis under near‐physiological conditions.

中文翻译：

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用于单分子FRET测量的天然哺乳动物蛋白质的位点特异性标记

不要FRET！我们描述了一种方法，该方法可以利用外部化学基团（例如荧光染料）对内源性细胞环境中产生的人类蛋白质进行快速位点特异性荧光标记，并提供一种实验策略，使标记的蛋白质适合在近乎生理条件下进行单分子FRET分析。