Biochimie ( IF 3.9 ) Pub Date : 2018-01-31 , DOI: 10.1016/j.biochi.2018.01.003 Chongsheng Xia , Ya Yang , Fanhua Kong , Qingli Kong , Changping Shan
Micro-RNAs have been reported to play crucial roles in a diversity of cellular processes such as cell proliferation, differentiation and development by regulating the expression of specific genes. They have also been shown to play vital roles in several diseases such as cancer. In the present study, we investigated the role of miR-143-3p in breast cancer. Our results showed that the expression of miR-143-3p is significantly downregulated in breast cancer cells. Upregulation of miR-143-3p inhibited the proliferation and migration of breast cancer cells. Conversely, inhibition of miR-143-3p promoted the proliferation of cancer cells. Bioinformatics analysis and other several experiments revealed MAPK7 as the potential target of miR-143-3p. Quantitative RT-PCR showed that the expression of MAPK7 correlated well with the expression of miR-143. Moreover, the inhibition of MAPK 7 in breast cancer cells abrogated the effects of miR-143 indicating that miR-143-3p-exerted effects on breast cancer are mediated by MAPK7. Takentogether, these results provide strong clues about the therapeutic potential of miR-143-3p in the treatment of breast cancer.
中文翻译:
MiR-143-3p通过调节MAPK7的表达抑制人乳腺癌细胞的增殖,细胞迁移和侵袭
据报道,微小RNA通过调节特定基因的表达在多种细胞过程中发挥关键作用,例如细胞增殖,分化和发育。还显示它们在多种疾病(例如癌症)中起着至关重要的作用。在本研究中,我们调查了miR-143-3p在乳腺癌中的作用。我们的结果表明,miR-143-3p的表达在乳腺癌细胞中显着下调。miR-143-3p的上调抑制了乳腺癌细胞的增殖和迁移。相反,抑制miR-143-3p可促进癌细胞的增殖。生物信息学分析和其他一些实验表明,MAPK7是miR-143-3p的潜在靶标。定量RT-PCR显示,MAPK7的表达与miR-143的表达密切相关。此外,乳腺癌细胞中MAPK 7的抑制作用消除了miR-143的作用,表明miR-143-3p对乳腺癌的作用是由MAPK7介导的。总而言之,这些结果为miR-143-3p在乳腺癌治疗中的治疗潜力提供了有力的线索。