In the article, we present the surface modification method of poly(l-lactide)/hydroxyapatite hybrid material using ultrashort pulse/femtosecond laser for tissue engineering application. Using femtosecond laser ablation we obtained 3D grooved structures at the composite surface with precisely controlled dimensions having 50–100 μm in width and a depth of 20 μm with porous bottom. Differential scanning calorimetry and XRD showed no significant influence of laser process on the supramolecular structure of the polymer in composite after modification. ATR analysis revealed partial surface “amorphisation” due to extremely high temperature gradient provided by laser pulses and relatively long crystallization time of PLLA. GPC revealed decrease in molecular weight of PLLA in the composite after laser modification. In addition biological tests were conducted. Human osteoblasts ATCC CRL-11372 were cultured on the laser-modified surface. Cytotoxicity and real time cell growth experiments showed no toxic effects of laser treated material on cells. This implies that femtosecond laser surface treatment is a promising method which potentially can be used in tissue engineering for scaffold modification and facilitating integration of bioresorbable implant and bone.

在本文中，我们介绍了聚（l-丙交酯/羟基磷灰石杂化材料，使用超短脉冲/飞秒激光进行组织工程应用。使用飞秒激光烧蚀，我们在复合材料表面获得了3D沟槽结构，具有精确控制的尺寸，其宽度为50–100μm，深度为20μm，底部为多孔。差示扫描量热法和XRD表明，改性后复合材料中激光工艺对聚合物的超分子结构没有显着影响。ATR分析表明，由于激光脉冲提供的极高温度梯度以及相对较长的PLLA结晶时间，导致部分表面“非晶化”。GPC显示激光改性后复合材料中PLLA的分子量降低。另外进行了生物学测试。在激光修饰的表面上培养人成骨细胞ATCC CRL-11372。细胞毒性和实时细胞生长实验表明，经激光处理的材料对细胞没有毒性作用。这意味着飞秒激光表面处理是一种有前途的方法，可潜在地用于组织工程中以进行支架修饰并促进生物可吸收植入物与骨骼的整合。