Inverse Fluorescence Correlation Spectroscopy (iFCS) is a variant of FCS where unlabeled particles in solution, or domains in membranes, displace their surrounding, signal-generating molecules and thereby generate fluctuations. iFCS has to date been applied to unlabeled as well as labeled particles and protein molecules, using fluorescence as well as Raman scattering as a signal source, in diffraction-limited detection volumes as well as in nano-wells, and on fixed surfaces as well as in lipid bilayers. This review describes these applications and discusses the potentials and pitfalls when using iFCS.

中文翻译：

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逆荧光相关光谱的潜力和缺陷

逆荧光相关光谱 (iFCS) 是 FCS 的一种变体，其中溶液中未标记的粒子或膜中的域会取代周围的信号生成分子，从而产生波动。迄今为止，iFCS 已应用于未标记以及标记的颗粒和蛋白质分子，使用荧光和拉曼散射作为信号源，在衍射限制的检测体积以及纳米孔中，以及固定表面以及在脂双层中。本评论描述了这些应用程序并讨论了使用 iFCS 时的潜力和缺陷。