Objective

Aiming to achieve bioactive dental resins that promote healing of surrounding tissues, we developed novel poly(2-hydroxyethyl methacrylate/trimethylolpropane trimethacrylate) (polyHEMA/TMPT) particles. These particles have been reported to be useful as a non-biodegradable carrier for fibroblast growth factor-2 (FGF-2) in vitro. The aim of this study was to evaluate the ability of an adhesive resin incorporating FGF-2-loaded polymer particles to promote tissue regeneration in vitro and in vivo.

Methods

Experimental adhesive resins were prepared by incorporating FGF-2-loaded polyHEMA/TMPT particles into a 4-META/MMA-based adhesive resin, and the release profiles of FGF-2 were evaluated. The proliferation of osteoblast-like cells in the eluate from cured experimental resin was assessed. When the experimental resin was implanted into rat calvaria defects, bone regeneration was evaluated by microcomputed tomography and histological observations.

Results

Sustained release of FGF-2 from the experimental resin was observed for 14 days. Eluate from the cured experimental resin significantly promoted the proliferation of osteoblast-like cells. Significantly greater bone regeneration was observed using the experimental resin compared with the control resin without FGF-2.

Significance

4-META/MMA-based adhesive resin incorporating FGF-2-loaded polymer particles is useful to promote tissue regeneration, suggesting that its application would be beneficial for root-end filling or the repair of fractured roots in cases with severely damaged periodontal tissue.

中文翻译：

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结合有FGF-2负载的聚合物颗粒的新型牙科树脂胶粘剂的开发，具有促进组织再生的能力

客观的

为了获得可促进周围组织愈合的生物活性牙科树脂，我们开发了新型聚（甲基丙烯酸2-羟乙酯/三羟甲基丙烷三甲基丙烯酸酯）（polyHEMA / TMPT）颗粒。据报道这些颗粒在体外可用作成纤维细胞生长因子-2（FGF-2）的不可生物降解的载体。这项研究的目的是评估掺有FGF-2的聚合物颗粒的粘合树脂在体外和体内促进组织再生的能力。

方法

通过将载有FGF-2-2的聚HEMA / TMPT颗粒掺入基于4-META / MMA的粘着树脂中来制备实验性粘着树脂，并评估FGF-2的释放曲线。评估了来自固化实验树脂的洗脱液中成骨细胞样细胞的增殖。当将实验树脂植入大鼠颅骨缺损中时，通过微型计算机断层扫描和组织学观察评估骨再生。

结果

观察到FGF-2从实验树脂中持续释放14天。固化的实验树脂中的洗脱液显着促进了成骨细胞样细胞的增殖。与不含FGF-2的对照树脂相比，使用实验树脂观察到明显更大的骨再生。

意义

掺有FGF-2的聚合物颗粒的基于4-META / MMA的粘合树脂可用于促进组织再生，表明在牙周组织严重受损的情况下，其应用对根端填充或修复断裂的根部将是有益的。