Systems for multigene delivery in mammalian cells, particularly in the context of genome engineering, have gained a lot of attention in biomolecular research and medicine. Initially these methods were based on RNA polymerase II promoters and were used for the production of protein complexes and for applications in cell biology such as reprogramming of somatic cells to stem cells. Emerging technologies such as CRISPR/Cas9-based genome engineering, which enable any alteration at the genomic level of an organism, require additional elements including U6-driven expression cassettes for RNA expression and homology constructs for designed genome modifications. For these applications, systems with high DNA capacity, flexibility and transfer rates are needed. In this article, we briefly give an update on some of recent strategies that facilitate multigene assembly and delivery into mammalian cells. Also, we review applications in various fields of biology that rely on multigene delivery systems.

用于哺乳动物细胞中多基因递送的系统，特别是在基因组工程的背景下，已在生物分子研究和医学中引起了很多关注。最初，这些方法基于RNA聚合酶II启动子，并用于蛋白质复合物的生产和细胞生物学中的应用，例如将体细胞重编程为干细胞。诸如基于CRISPR / Cas9的基因组工程等新兴技术可实现在生物体基因组水平上的任何改变，它需要其他元素，包括用于RNA表达的U6驱动表达盒和用于设计基因组修饰的同源构建体。对于这些应用，需要具有高DNA容量，灵活性和转移速率的系统。在本文中，我们简要介绍了一些近期策略的最新进展，这些策略促进了多基因组装和向哺乳动物细胞的传递。此外，我们还将回顾依赖于多基因递送系统的生物学各个领域的应用。