A plant microbiome includes a microbial community that typically interacts extensively with a plant. The plant microbiome can survive either inside or outside of plant tissues, performing various plant beneficial activities including biocontrol of potential phytopathogens and promotion of plant growth. An important part of the plant microbiome includes plant growth-promoting bacteria (PGPB) that commonly reside in the rhizosphere and phyllosphere, and as endophytic bacteria (inside of plant tissues). As new plant microbiome-manipulating strategies have emerged in recent years, we have critically reviewed relevant literature, chiefly from the last decade. We have analysed and compared the rhizosphere, phyllosphere and endosphere as potential ecosystems for manipulation, in order to improve positive interactions with the plant. In addition, many studies on the bioengineering of the endophyte microbiome and its potential impact on the core microbiome were analysed with respect to five different strategies, including host mediated and multi-generation microbiome selection, inoculation into soil and rhizosphere, inoculations into seeds or seedlings, tissue atomisation and direct injection into tissues or wounds. Finally, microbiome engineering presents a feasible strategy to solve multiple agriculture-associated problems in an eco-friendly way.

植物微生物组包括通常与植物广泛相互作用的微生物群落。植物微生物组可以在植物组织内部或外部生存，执行各种植物有益的活动，包括对潜在植物病原体的生物控制和促进植物生长。植物微生物组的重要组成部分包括植物生长促进细菌（PGPB），它们通常位于根际和叶际，并作为内生细菌（在植物组织内部）存在。由于近年来出现了新的植物微生物组操纵策略，因此我们主要从最近十年开始对相关文献进行了严格的审查。我们已经分析并比较了根际，根际和内层作为潜在的可操纵生态系统，以改善与植物的积极相互作用。此外，从五个不同的策略，分析了许多有关内生菌微生物组生物工程及其对核心微生物组的潜在影响的研究，包括宿主介导的和多代微生物组选择，接种到土壤和根际，接种到种子或幼苗，组织雾化等五种策略并直接注射到组织或伤口中。最后，微生物组工程学提出了一种可行的策略，以一种生态友好的方式解决与农业相关的多个问题。组织雾化并直接注射到组织或伤口中。最后，微生物组工程学提出了一种可行的策略，以一种生态友好的方式解决与农业相关的多个问题。组织雾化并直接注射到组织或伤口中。最后，微生物组工程学提出了一种可行的策略，以一种生态友好的方式解决与农业相关的多个问题。