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Antagonistic Bacillus spp. reduce blast incidence on rice and increase grain yield under field conditions
Microbiological Research ( IF 6.7 ) Pub Date : 2018-01-31 , DOI: 10.1016/j.micres.2018.01.009
Afroz Rais , Muhammad Shakeel , Kamran Malik , Fauzia Yusuf Hafeez , Humaira Yasmin , Saqib Mumtaz , Muhammad Nadeem Hassan

Rice blast is a severe threat for agricultural production. Plant growth promoting rhizobacteria could be suitable biocontrol agents to reduce the disease incidence. In this study, Bacillus spp. KFP-5, KFP-7, KFP-17 significantly reduced disease severity by 40–52% with grain yield of 3.2–3.9 t ha−1 in two rice varieties i.e., basmati super and basmati 385. Bacillus spp. significantly colonized the rice rhizosphere with a cell population of 2.40E+06–5.6E+07CFU. Rice plants treated with antagonistic bacterial suspension followed by challenge inoculation with P. oryzae were found to have higher activities of antioxidant enzymes such as superoxide dismutase (308–266 Ug−1 FW), peroxidase (change in absorbance (ΔA) = 0.20–0.71 min−1 g−1 FW), polyphenol oxidase (ΔA = 0.29–0.58 min−1 g−1 FW) and phenylalanine ammonia lyase (ΔA = 0.32–0.59 min−1 g−1 FW). A consistency in the performance of strains was observed in the consecutive years 2013–2014. These findings suggest that indigenous Bacillus spp. could be a potential bio-inoculum for rice to control blast diseases and enhance yield.



中文翻译:

拮抗芽孢杆菌属。在田间条件下减少稻瘟病发生率并提高谷物产量

稻瘟病是对农业生产的严重威胁。促进根瘤菌生长的植物可能是降低疾病发生率的合适生物防治剂。在这项研究中,芽孢杆菌属。KFP-5,KFP-7,KFP-17显著由40-52%降低疾病的严重程度与3.2-3.9籽粒产量吨公顷-1两个水稻品种即巴斯马蒂超级巴斯马蒂385.芽孢杆菌属。水稻根际上的菌落数量显着增加,为2.40E + 06–5.6E + 07CFU。水稻植株经过拮抗细菌悬浮液处理后再挑战米曲霉接种后发现具有较高的抗氧化酶活性,如超氧化物歧化酶(308–266 Ug -1 FW),过氧化物酶(吸光度变化(ΔA)= 0.20–0.71 min -1  g -1  FW),多酚氧化酶(ΔA= 0.29–0.58 min -1  g -1  FW)和苯丙氨酸氨裂解酶(ΔA= 0.32–0.59最小-1  g -1  FW)。在2013-2014年连续的几年中,观察到菌株性能的一致性。这些发现表明,土著芽孢杆菌属。可能是水稻控制稻瘟病和提高产量的潜在生物菌落。

更新日期:2018-01-31
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