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Liquid CO2 in Centrifuge Tubes: Separation of Chamazulene from Blue Tansy (Tanacetum annuum) Oil via Extraction and Thin-Layer Chromatography
Journal of Chemical Education ( IF 3 ) Pub Date : 2018-01-24 00:00:00 , DOI: 10.1021/acs.jchemed.7b00610
Bruce W. Baldwin 1 , Thomas S. Kuntzleman 1
Affiliation  

The separation of chamazulene from hydrophilic contaminants present in blue tansy oil provides a visually engaging example of two common techniques: extraction and thin-layer chromatography (TLC). This application uses liquid CO2 as a lipophilic solvent to pull a brilliant blue hydrocarbon molecule, chamazulene, out of or through a common hydrophilic material, cellulose. Yellow-green, hydrophilic components remain in a cotton ball or at the lower end of a paper strip. In dramatic fashion, the separation of colored components of blue tansy oil is accomplished with an environmentally sustainable solvent, CO2, and an innocuous support material, cellulose. We believe this is the first reported use of liquid CO2 in centrifuge tubes to conduct TLC.

中文翻译:

离心管中的液态CO 2:通过萃取和薄层色谱法从蓝艾油中分离去甲苯磺灵

从蓝色艾菊油中存在的亲水性污染物中分离出甲磺灵,这是两种常见技术的引人注目的例子:萃取和薄层色谱法(TLC)。此应用程序使用液态CO 2作为亲脂性溶剂,从一种或多种常见的亲水性材料纤维素中拉出亮蓝色的烃分子Chamazulene。黄绿色的亲水性成分保留在棉球中或纸条的下端。蓝色艾菊油中有色成分的分离以一种环境可持续的溶剂CO 2和无害的支撑材料纤维素来完成。我们相信这是首次报道在离心管中使用液态CO 2进行薄层色谱法。
更新日期:2018-01-24
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