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Making Enzyme Kinetics Dynamic via Simulation Software
Journal of Chemical Education ( IF 3 ) Pub Date : 2018-01-24 00:00:00 , DOI: 10.1021/acs.jchemed.7b00350 Jeffrey P. Potratz 1
Journal of Chemical Education ( IF 3 ) Pub Date : 2018-01-24 00:00:00 , DOI: 10.1021/acs.jchemed.7b00350 Jeffrey P. Potratz 1
Affiliation
An interactive classroom demonstration that enhances students’ knowledge of steady-state and Michaelis–Menten enzyme kinetics is described. The instructor uses a free version of professional-quality KinTek Explorer simulation software and student input to construct dynamic versions of three static hallmark images commonly used to introduce enzyme kinetic concepts. The software, with its ability to change experimental conditions and immediately observe the effects on the kinetics of a system, allows students to be more aware of the experimental conditions that must exist for the assumptions of steady-state and Michaelis–Menten kinetics to be valid. Students report an increased understanding of the interplay between hallmark concentration versus time and rate versus concentration images. After the demonstration, students are prepared for data generation and analysis in a lab experiment focused on the steady-state. Additionally, they can begin independent simulation exercises using the software and are ready for discussions on pre-steady-state kinetics.
中文翻译:
通过仿真软件动态化酶动力学
描述了一个互动的课堂演示,该演示可以增强学生对稳态和米利斯-门腾酶动力学的了解。讲师使用专业质量的KinTek Explorer模拟软件的免费版本和学生输入来构造三个静态标记图像的动态版本,这些图像通常用于引入酶动力学概念。该软件具有更改实验条件并立即观察对系统动力学的影响的能力,使学生可以更清楚地了解对于稳态和Michaelis-Menten动力学假设必须存在的实验条件。 。学生们报告了对标记浓度与时间,速率与浓度图像之间相互作用的更多了解。示范之后 学生将准备在稳态下进行的实验室实验中进行数据生成和分析。此外,他们可以使用该软件开始独立的模拟练习,并准备进行关于稳态前动力学的讨论。
更新日期:2018-01-24
中文翻译:
通过仿真软件动态化酶动力学
描述了一个互动的课堂演示,该演示可以增强学生对稳态和米利斯-门腾酶动力学的了解。讲师使用专业质量的KinTek Explorer模拟软件的免费版本和学生输入来构造三个静态标记图像的动态版本,这些图像通常用于引入酶动力学概念。该软件具有更改实验条件并立即观察对系统动力学的影响的能力,使学生可以更清楚地了解对于稳态和Michaelis-Menten动力学假设必须存在的实验条件。 。学生们报告了对标记浓度与时间,速率与浓度图像之间相互作用的更多了解。示范之后 学生将准备在稳态下进行的实验室实验中进行数据生成和分析。此外,他们可以使用该软件开始独立的模拟练习,并准备进行关于稳态前动力学的讨论。