This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from an optics perspective. As such, emphasis is placed on the advantages that LS microscope configurations present, given the degree of freedom gained by uncoupling the excitation and detection arms. The new imaging properties are first highlighted in terms of optical parameters and how these have enabled several biomedical applications. Then, the basics are presented for understanding how a LS microscope works. This is followed by a presentation of a tutorial for LS microscope designs, each working at different resolutions and for different applications. Then, based on a numerical Fourier analysis and given the multiple possibilities for generating the LS in the microscope (using Gaussian, Bessel, and Airy beams in the linear and nonlinear regimes), a systematic comparison of their optical performance is presented. Finally, based on advances in optics and photonics, the novel optical implementations possible in a LS microscope are highlighted.

中文翻译：

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光片显微镜：教程

本文旨在从光学角度对光片 (LS) 显微镜进行全面回顾。因此，考虑到通过分离激发臂和检测臂获得的自由度，重点放在 LS 显微镜配置存在的优势上。新的成像特性首先在光学参数方面突出显示，以及这些特性如何实现了多种生物医学应用。然后，介绍基础知识以了解 LS 显微镜的工作原理。接下来是介绍 LS 显微镜设计的教程，每个教程都以不同的分辨率和不同的应用工作。然后，基于数值傅立叶分析并给出在显微镜中生成 LS 的多种可能性（在线性和非线性区域中使用高斯、贝塞尔和艾里光束），系统地比较了它们的光学性能。最后，基于光学和光子学的进步，重点介绍了 LS 显微镜中可能的新型光学实现。