The artificial bilayer single channel recording technique is commonly used to observe the detailed physiological properties of various ion channel proteins. It permits easy control of the solution and membrane lipid composition, and is also compatible with pharmacological screening devices. However, its use is limited due to low measurement efficiency. Here, we developed a novel artificial bilayer single channel recording technique in which solubilized ion channel proteins immobilized on a gold nano-electrode are directly incorporated into a lipid bilayer at the same time as the bilayer is formed at the tip of it on coming in contact with an aqueous–oil interface. Using this technique, we measured the single channel currents of several types of channels including KcsA, MthK, hBK and P2X4. This technique requires only one action to simultaneously form the bilayers and reconstitute the channels into the membranes. This simplicity greatly increases the measurement efficiency and allows the technique to potentially be combined with high-throughput screening devices.

中文翻译：

---

用于单个离子通道记录的金纳米电极†

人工双层单通道记录技术通常用于观察各种离子通道蛋白的详细生理特性。它可以轻松控制溶液和膜脂质成分，并且与药理筛选设备兼容。但是，由于测量效率低，其使用受到限制。在这里，我们开发了一种新颖的人工双层单通道记录技术，该技术将固定在金纳米电极上的可溶解离子通道蛋白直接掺入脂质双层中，同时在接触时在其尖端形成双层具有水-油界面。使用这种技术，我们测量了包括KcsA，MthK，hBK和P2X4在内的几种类型通道的单通道电流。该技术仅需一个动作即可同时形成双层并重新构成进入膜的通道。这种简单性大大提高了测量效率，并使该技术有可能与高通量筛选设备结合使用。