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Myoglobin immobilized on mesoporous carbon foam in a hydrogel (selep) dispersant for voltammetric sensing of hydrogen peroxide
Microchimica Acta ( IF 5.7 ) Pub Date : 2018-01-19 , DOI: 10.1007/s00604-017-2654-9
Mojtaba Jahanbakhshi

AbstractMesoporous carbon foam (MCF) was prepared by via the Pechini method which is facile and template-free. The MCF was characterized by field emission scanning electron microscopy, transmission electron microscopy, X-ray diffraction and BET surface area analysis. Afterwards, the MCF was dispersed in the natural hydrogel salep to give a composite. Finally, myoglobin was immobilized on the composite and then placed on a glassy carbon electrode (GCE). The modified GCE gives a distinct quasi-reversible redox peak during electroreduction of hydrogen perxide (H2O2). The estimated electron transfer coefficient (α) and the heterogeneous electron transfer rate constant (ks) for redox process of Mb are 0.54 and 2.25 s−1, respectively. The sensor, best operated at −0.2 V (vs. Ag/AgCl), responds to H2O2 in the 1.0 to 80 μM H2O2 concentration range, with a 180 nM limit of detection (at S/N ratio of 3). The technique was applied to the determination of H2O2 in spiked fetal bovine serum samples. Graphical abstractMesoporous carbon foam (MCF) synthesis, dispersion in Salep solution, preparing the Salep-MCF composite (S-MCF), immobilizing the Mb at S-MCF and preparing Mb/S-MCF composite, Casting the Mb/S-MCF on electrode surface to prepare Mb/S-MCF/GCE and electrochemical behavior of biosensor.

中文翻译:

肌红蛋白固定在水凝胶(selep)分散剂中的介孔碳泡沫上,用于过氧化氢的伏安检测

摘要 采用Pechini法制备介孔碳泡沫(MCF),该方法简便、无模板。MCF 的特点是场发射扫描电子显微镜、透射电子显微镜、X 射线衍射和 BET 表面积分析。然后,将 MCF 分散在天然水凝胶 salep 中以得到复合材料。最后,将肌红蛋白固定在复合材料上,然后放置在玻璃碳电极 (GCE) 上。改进的 GCE 在过氧化氢 (H2O2) 的电还原过程中给出了明显的准可逆氧化还原峰。Mb 氧化还原过程的估计电子转移系数 (α) 和异质电子转移速率常数 (ks) 分别为 0.54 和 2.25 s-1。传感器最好在 -0.2 V(相对于 Ag/AgCl)下运行,对 1.0 至 80 μM H2O2 浓度范围内的 H2O2 做出响应,检测限为 180 nM(信噪比为 3)。该技术用于测定加标胎牛血清样品中的 H2O2。图形摘要介孔碳泡沫 (MCF) 合成,在 Salep 溶液中分散,制备 Salep-MCF 复合材料 (S-MCF),将 Mb 固定在 S-MCF 上并制备 Mb/S-MCF 复合材料,将 Mb/S-MCF 浇铸在电极表面制备 Mb/S-MCF/GCE 和生物传感器的电化学行为。
更新日期:2018-01-19
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