This review presents some of the hottest topics in biotechnological applications: proteases in biocatalysis. Obviously, one of the most relevant areas of application is in the hydrolysis of proteins in food technology, and that has led to a massive use on proteomics. The aim is to identify via peptide maps the different proteins obtained after a specific protease hydrolysis. However, concepts like degradomics are also taking on a more relevant importance in the use and study of proteases and will also be discussed. Other protease applications, as seem in cleaning (detergent development), the pharmaceutical industry, and in fine chemistry, will be analyzed. This review progresses from basic areas such as protease classification to a discussion of the preparation of protease‐immobilized biocatalysts, considering the different problems raised by the use of immobilized proteases due to the peculiar features of the substrates, usually large macromolecules. Production of bioactive peptides via limited hydrolysis of proteins will occupy an important place in this review.

中文翻译：

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蛋白酶在食品技术中的生物技术应用

这篇评论提出了一些在生物技术应用中最热门的话题：生物催化中的蛋白酶。显然，最相关的应用领域之一是食品技术中蛋白质的水解，这导致了蛋白质组学的广泛应用。目的是通过肽图鉴定在特定蛋白酶水解后获得的不同蛋白质。然而，诸如降解组学的概念在蛋白酶的使用和研究中也越来越重要，并且还将进行讨论。将分析蛋白酶在清洁（洗涤剂开发），制药行业和精细化学中的其他蛋白酶应用。这篇综述从诸如蛋白酶分类的基本领域发展到了固定蛋白酶生物催化剂的讨论，考虑到由于底物（通常是大分子）的特殊特征，使用固定化蛋白酶引起的不同问题。通过蛋白质的有限水解来生产生物活性肽将在本综述中占据重要位置。