A novel polysaccharide (WGP) was purified from crude wheat germ polysaccharide by Sephacryl S-500HRgel filtration. The molecular weight of WGP was determined as 4.89 × 10⁶ Da and consisted of arabinose, xylose, glucose, and galactose. Methylation analysis and 1D/2D nuclear magnetic resonance was used to analyze the structural characterization of WGP. WGP was mainly a backbone composed of (1 → 4)-linked-β-d-Xylp (19.01%) and (1 → 3, 4)-linked-β-d-Xylp (26.27%) residues, which was branched of (1 → 5)-linked α-l-Araf (28.09%) and (1 → 3,6)-linked β-d-Galp (12.11%) with β-d-Glcp (14.52%) as terminal unit. The calculated values of Turbiscan stability indexes suggested that WGP (0.1–0.5 mg/mL) is a stable system. Microrheology results showed that WGP can form gel behavior when the concentration of WGP ranges from 0.1 to 3 mg/mL. Results of in vitro assays showed that WGP could cause the proliferation of RAW264.7 macrophages, upregulating the release of TNF-α and IL-8 in the lymphocytes.

中文翻译：

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小麦胚芽同质多糖的结构变异和微流变特性

通过Sephacryl S-500HRgel过滤从小麦胚芽多糖中纯化出一种新型多糖（WGP）。WGP的分子量确定为4.89×10 ⁶ Da，由阿拉伯糖，木糖，葡萄糖和半乳糖组成。甲基化分析和1D / 2D核磁共振被用来分析WGP的结构表征。WGP主要是由（1→4）-β - d - Xylp（19.01％）和（1→3，4）-β - d - Xylp（26.27％）残基组成的骨架，由（1→5）联结的α- 1-阿拉法（28.09％）和（1→3,6）联结的β- d-带有（β→ d-Glcp（14.52％）作为终端设备。Turbiscan稳定性指数的计算值表明，WGP（0.1–0.5 mg / mL）是一个稳定的系统。微流变学结果表明，当WGP的浓度在0.1至3 mg / mL范围内时，WGP可以形成凝胶行为。体外试验结果表明，WGP可以引起RAW264.7巨噬细胞增殖，从而上调淋巴细胞中TNF-α和IL-8的释放。