The analysis of pomegranate phenolic compounds belonging to different classes in different fruit parts was performed by high-performance liquid chromatography coupled with photodiode array and mass spectrometry detection. Two different separation methods were optimized for the analysis of anthocyanins and hydrolyzable tannins along with phenolic acids and flavonoids. Two C₁₈ columns, core–shell and fully porous particle stationary phases, were used. The parameters for separation of phenolic compounds were optimized considering chromatographic resolution and analysis time. Thirty-five phenolic compounds were found, and 28 of them were tentatively identified as belonging to four different phenolic compound classes; namely, anthocyanins, phenolic acids, hydrolyzable tannins, and flavonoids. Quantitative analysis was performed with a mixture of nine phenolic compounds belonging to phenolic compound classes representative of pomegranate. The method was then fully validated in terms of retention time precision, expressed as the relative standard deviation, limit of detection, limit of quantification, and linearity range. Phenolic compounds were analyzed directly in pomegranate juice, and after solvent extraction with a mixture of water and methanol with a small percentage of acid in peel and pulp samples. The accuracy of the extraction method was also assessed, and satisfactory values were obtained. Finally, the method was used to study identified analytes in pomegranate juice, peel, and pulp of six different Italian varieties and one international variety. Differences in phenolic compound profiles among the different pomegranate parts were observed. Pomegranate peel samples showed a high concentration of phenolic compounds, ellagitannins being the most abundant ones, with respect to pulp and juice samples for each variety. With the same samples, total phenols and antioxidant activity were evaluated through colorimetric assays, and the results were correlated among them.

采用高效液相色谱法结合光电二极管阵列和质谱检测技术，对不同水果部位的石榴酚类化合物进行了分析。优化了两种不同的分离方法，用于分析花色苷和可水解单宁以及酚酸和类黄酮。两个C ₁₈使用了柱，核-壳和全多孔颗粒固定相。考虑到色谱分离度和分析时间，优化了用于分离酚类化合物的参数。共发现了35种酚类化合物，其中28种被初步鉴定为属于4种不同的酚类化合物。即花青素，酚酸，可水解单宁和类黄酮。用属于代表石榴的酚类化合物的九种酚类化合物的混合物进行定量分析。然后，该方法在保留时间精度方面得到了充分验证，该保留时间表示为相对标准偏差，检测限，定量限和线性范围。直接在石榴汁中分析酚类化合物，并在果皮和果肉样品中用水和甲醇以及少量酸的混合物萃取溶剂后。还评估了提取方法的准确性，并获得了令人满意的值。最后，该方法用于研究六个意大利不同品种和一个国际品种的石榴汁，果皮和果肉中已鉴定的分析物。观察到在不同的石榴部分之间酚类化合物分布的差异。石榴皮样品显示出高浓度的酚类化合物，其中鞣花单宁含量最高，相对于每个品种的果肉和果汁样品。对于相同的样品，通过比色法评估了总酚和抗氧化剂的活性，并将结果关联起来。