Applying ultrasound (US) to drug delivery and disease therapy is important work. Sonodynamic therapy (SDT)—a comprehensive therapy using US and a sonosensitizer—exhibits antineoplastic activity in many tumors. In this study, we investigated the feasibility of using a new sonosensitizer (sinoporphyrin sodium, DVDMS) loaded into liposome–microbubble complexes (DLMBs) as a possible candidate to enhance SDT against breast cancer. DLMBs were synthesized via the biotin–avidin linkage and confirmed to have good US response. US-induced cavitation played a key role to trigger a boosted payload release from DLMBs and improve the cellular uptake and intratumoral diffusion of DVDMS to realize better SDT effect. The combination of DLMBs and US treatment resulted in significant changes to cell morphology, mitochondria damage, and cell apoptosis in vitro. In vivo, the combined treatment markedly inhibited tumor growth, which appeared to result from increased apoptosis and reduced proliferation activity. The significant increase in the antitumor effect of DLMBs plus US suggests their potential use as a new approach to promote the killing activity of SDT against breast cancer.

中文翻译：

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超声触发脂质体-微泡复合物中的卟啉钠的释放及其对乳腺癌的声动力学毒性增强

将超声（US）应用于药物输送和疾病治疗是重要的工作。声动力学疗法（SDT）是一种使用US和声敏剂的综合疗法，在许多肿瘤中均具有抗肿瘤活性。在这项研究中，我们调查了使用脂质体-微泡复合物（DLMBs）中装载的新型声敏剂（卟啉钠，DVDMS）作为增强SDT对抗乳腺癌的可能候选方案的可行性。DLMB通过生物素-亲和素连接合成，并被证实具有良好的美国反应。美国引起的空化起着关键作用，触发了DLMB的有效载荷释放的增加，并改善了DVDMS的细胞吸收和肿瘤内扩散，从而实现了更好的SDT效果。DLMB和US治疗的结合导致细胞形态，线粒体损伤和细胞凋亡发生重大变化体外。在体内，联合治疗显着抑制了肿瘤的生长，这似乎是由于凋亡增加和增殖活性降低所致。DLMBs和US的抗肿瘤作用显着提高，表明它们潜在地用作增强SDT对乳腺癌的杀伤活性的新方法。