The low-cost Open qPCR instrument can be used for different tasks in the aptamer selection process: quantification of DNA, cycle course optimization, screening, and final binding characterization. We have selected aptamers against whole Drosophila C virus (DCV) particles and recombinant epidermal growth factor receptor (EGFR). We performed systematic evolution of ligands by exponential enrichment (SELEX) using the Open qPCR to optimize each amplification step. The Open qPCR instrument identified the best aptamer candidate. The Open qPCR has the capacity to perform melt curves, and we used this function to perform thermofluorimetric analysis (TFA) to quantify target-aptamer binding. We confirmed target-aptamer binding using flow cytometry. A sandwich type luminescence bioassay based on our anti-DCV aptamer was sensitive to DCV and did not respond to a related virus, demonstrating that our selected anti-DCV aptamer can be used to specifically detect DCV.

中文翻译：

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开放式qPCR仪器在体外选择针对表皮生长因子受体和果蝇C病毒的DNA适体中的应用

低成本Open qPCR仪器可用于适体选择过程中的不同任务：DNA定量，循环过程优化，筛选和最终结合表征。我们选择了针对整个果蝇的适体C病毒（DCV）颗粒和重组表皮生长因子受体（EGFR）。我们使用开放qPCR通过指数富集（SELEX）进行了配体的系统进化，以优化每个扩增步骤。Open qPCR仪器确定了最佳的适体候选物。Open qPCR具有执行熔解曲线的能力，我们使用此功能进行热荧光分析（TFA）以量化靶标-适体结合。我们使用流式细胞仪确认了靶标-适体结合。基于我们的抗DCV适体的三明治型发光生物测定法对DCV敏感，对相关病毒没有反应，表明我们选择的抗DCV适体可用于特异性检测DCV。