New generations of biorefinery combine innovative biomass waste resources from different origins, chemical extraction and/or synthesis of biomaterials, biofuels, and bioenergy via green and sustainable processes. From the very beginning, identifying and evaluating all potentially high value-added chemicals that could be removed from available renewable feedstocks requires robust, efficient, selective, reproducible, and benign analytical approaches. With this in mind, green and sustainable separation of natural products from agro-industrial waste is clearly attractive considering both socio-environmental and economic aspects. In this paper, the concepts of green and sustainable separation of natural products will be discussed, highlighting the main studies conducted on this topic over the last 10 years. The principal analytical techniques (such as solvent, microwave, ultrasound, and supercritical treatments), by-products (e.g., citrus, coffee, corn, and sugarcane waste) and target compounds (polyphenols, proteins, essential oils, etc.) will be presented, including the emerging green and sustainable separation approaches towards bioeconomy and circular economy contexts.

新一代生物精炼厂通过绿色和可持续的工艺将不同来源的创新生物质废物资源、生物材料、生物燃料和生物能源的化学提取和/或合成结合起来。从一开始，识别和评估所有可以从可用的可再生原料中去除的潜在高附加值化学品就需要稳健、高效、选择性、可重复和良性的分析方法。考虑到这一点，考虑到社会环境和经济方面，从农工业废物中绿色和可持续地分离天然产物显然具有吸引力。本文将讨论天然产物的绿色和可持续分离的概念，重点介绍过去 10 年来针对该主题进行的主要研究。主要分析技术（如溶剂、微波、超声波和超临界处理）、副产品（如柑橘、咖啡、玉米和甘蔗废料）和目标化合物（多酚、蛋白质、精油等）将在介绍了生物经济和循环经济背景下新兴的绿色和可持续分离方法。