A strategy for biocatalyst immobilization in flow directly from the crude cell extract is described. The efficiency and the stability of the immobilized enzyme were demonstrated during the asymmetric reduction of a range of ketones. The cascade two-step chemo-enzymatic preparation of chiral epoxides was possible through the initial ketone bioreduction to an intermediate halohydrin followed by its intramolecular cyclization.

中文翻译：

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直接从粗细胞提取物中 快速，选择性和稳定地固定HaloTag- Lb ADH，以连续生物催化生产手性醇和环氧化物†

描述了直接从粗细胞提取物中固定流中生物催化剂的策略。在一系列酮的不对称还原过程中，证明了固定化酶的效率和稳定性。通过最初的酮生物还原为中间卤代醇，然后进行分子内环化，可以实现手性环氧化物的级联两步化学酶法制备。