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Improvement of nutritional components and in vitro antioxidative properties of soy-powder yogurts using Lactobacillus plantarum
Journal of Food and Drug Analysis ( IF 3.6 ) Pub Date : 2018-07-01 , DOI: 10.1016/j.jfda.2017.12.003
Jin Hwan Lee 1 , Chung Eun Hwang 2 , Eun Ju Cho 3 , Yeong Hun Song 4 , Su Cheol Kim 2 , Kye Man Cho 2
Affiliation  

This research was the first to demonstrate changes in nutritional compositions (isoflavone and CLA) from the 50% methanol extracts of soy-powder milk (SPM) and soy-powder yogurt (SPY) through fermentation using Lactobacillus plantarum S48 and P1201 strains. The radical scavenging activities and protective effects against oxidative stress in LLC-PK1 cells were also investigated. The average physicochemical characteristics including acidity and viable cell number as well as β-glucosidase activity increased with 0.2 → 0.7%, 7.5 → 9.8 log cfu/mL, and 0.0 3 → 1.75 U/g in SPYs. Total average isoflavones were considerably reduced (3180.3 → 2018.3 μg/g) with the increase of aglycone contents (191.8 → 770.2 μg/g), especially, daidzein exhibited the most remarkable increase rate (98.6 → 460.9 μg/g; > 4.8 times) during fermentation. The CLA and total phenolics also increased with significant differences (ND → 1.6 mg/g; 2.4 → 3.6 mg/GAE/g) between SPM and SPY. Interestingly, the cis-9, trans-11 CLA showed approximately 90% in total content. Moreover, the scavenging capacities against three radicals markedly increased with about 30% in SPYs, as the following order: ABTS > hydroxyl > DPPH. The protective effects on oxidative stress (pyrogallol: O2-, SNP: NO, and SIN-1: ONOO-) were also observed high cell viabilities (>10%) under LLC-PK1 cellular system. Our results suggest that SPY may be utilized as a potent source regarding natural antioxidants and beneficial components for health food and medical uses.

中文翻译:

植物乳杆菌改善豆粉酸奶的营养成分和体外抗氧化特性

这项研究首次证明了通过使用植物乳杆菌 S48 和 P1201 菌株发酵,豆粉奶 (SPM) 和豆粉酸奶 (SPY) 的 50% 甲醇提取物的营养成分(异黄酮和 CLA)发生了变化。还研究了 LLC-PK1 细胞的自由基清除活性和对氧化应激的保护作用。SPYs 的平均理化特性包括酸度和活细胞数以及 β-葡萄糖苷酶活性随着 0.2 → 0.7%、7.5 → 9.8 log cfu/mL 和 0.0 3 → 1.75 U/g 的增加而增加。随着苷元含量的增加(191.8 → 770.2 μg/g),总平均异黄酮显着降低(3180.3 → 2018.3 μg/g),尤其是黄豆苷元表现出最显着的增加率(98.6 → 460.9 μg/g;> 4.8倍)在发酵过程中。CLA 和总酚类物质也随着 SPM 和 SPY 之间的显着差异(ND → 1.6 mg/g;2.4 → 3.6 mg/GAE/g)增加。有趣的是,cis-9、trans-11 CLA 的总含量约为 90%。此外,SPYs 对三种自由基的清除能力显着增加约 30%,顺序如下:ABTS > 羟基 > DPPH。在 LLC-PK1 细胞系统下,还观察到对氧化应激(邻苯三酚:O2-、SNP:NO 和 SIN-1:ONOO-)的保护作用具有高细胞活力(>10%)。我们的研究结果表明,SPY 可作为天然抗氧化剂和有益成分的有效来源,用于保健食品和医疗用途。trans-11 CLA 的总含量约为 90%。此外,SPYs 对三种自由基的清除能力显着增加约 30%,顺序如下:ABTS > 羟基 > DPPH。在 LLC-PK1 细胞系统下,还观察到对氧化应激(邻苯三酚:O2-、SNP:NO 和 SIN-1:ONOO-)的保护作用具有高细胞活力(>10%)。我们的研究结果表明,SPY 可作为天然抗氧化剂和有益成分的有效来源,用于保健食品和医疗用途。trans-11 CLA 的总含量约为 90%。此外,SPYs 对三种自由基的清除能力显着增加约 30%,顺序如下:ABTS > 羟基 > DPPH。在 LLC-PK1 细胞系统下,还观察到对氧化应激(邻苯三酚:O2-、SNP:NO 和 SIN-1:ONOO-)的保护作用具有高细胞活力(>10%)。我们的研究结果表明,SPY 可作为天然抗氧化剂和有益成分的有效来源,用于保健食品和医疗用途。10%) 在 LLC-PK1 蜂窝系统下。我们的研究结果表明,SPY 可作为天然抗氧化剂和有益成分的有效来源,用于保健食品和医疗用途。10%) 在 LLC-PK1 蜂窝系统下。我们的研究结果表明,SPY 可作为天然抗氧化剂和有益成分的有效来源,用于保健食品和医疗用途。
更新日期:2018-07-01
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