Food allergies are increasingly recognized as a major healthcare concern. In order to sensitively and specifically detect allergies from blood samples of at-risk allergic patients, an effective magnetic fluorescence sensing platform (EMFP) was constructed. The EMFP incorporated hollow mesoporous silica nanospheres (HMNs) to amplify signal from the target IgE in addition to magnetic nanoparticles (MNPs) to capture and separate the target IgE. The application of EMFP to immunoassays indicated a detection limit of 0.0159 ng mL⁻¹ for low concentration specific immunoglobulin E (sIgE) against purified shellfish Metapenaeus ensis (Meta. E.) allergens, which is 15 fold more sensitive than the commercially available Food and Drug Administration-approved analyzers. Notably, EMFP was specific for the targeted sIgE even with interference by other sIgEs. In addition, the detection time is only 75 min, considerably faster than current commercial ELISA kits for IgE assays. Together, these results demonstrated that EMFP has excellent sensitivity and selectivity for the rapid detection of sIgE. The method thus exhibits potential toward the rapid monitoring of sIgE against Meta. E. allergens in clinical application.

中文翻译：

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一种选择性和灵敏的纳米传感器，用于荧光检测人血清中纯化过敏原的特异性 IgE

食物过敏越来越被认为是一个主要的医疗保健问题。为了灵敏、特异地检测高危过敏患者血液样本中的过敏情况，构建了一个有效的磁荧光传感平台（EMFP）。EMFP 结合了中空介孔二氧化硅纳米球 (HMN) 以放大来自目标 IgE 的信号，以及磁性纳米粒子 (MNP) 以捕获和分离目标 IgE。EMFP 在免疫测定中的应用表明低浓度特异性免疫球蛋白 E (sIgE) 对纯化的贝类Metapenaeus ensis的检测限为 0.0159 ng mL ^-1(Meta. E.) 过敏原，比市售食品和药物管理局批准的分析仪敏感 15 倍。值得注意的是，EMFP 对目标 sIgE 具有特异性，即使受到其他 sIgE 的干扰。此外，检测时间仅为 75 分钟，比目前用于 IgE 检测的商业 ELISA 试剂盒快得多。总之，这些结果表明 EMFP 对 sIgE 的快速检测具有出色的灵敏度和选择性。因此，该方法显示出快速监测 sIgE 对 Meta 的潜力。E. 临床应用中的过敏原。