To acquire microbial consortia with effectively precedent degradation of lignin, samples obtained from rotten trunks, rotten stumps and soil near it were screened and isolated after generations of subculture. The dynamic change illustrated that their community structures were affected by pH and tended to be stable after 6 days’ cultivation. The desired one, named DM-1, was gained through successive cultivation for over 5 generations, whose high selectivity in lignin degradation was observed within 16 days’ cultivation (SV = 2.78). Meanwhile, a remarkable reduction in the fiber crystallinity of tree trimmings (10.35%) resulted from the bio-degradation of DM-1, displayed a greater exposure of cellulose by selective removal of lignin. The diversity analysis of DM-1 was investigated by PCR amplification and 16S rDNA sequencing, indicated that mesorhizobium, cellulosimicrobium, pandoraea, achromobacter and stenotrophomones were the predominant genera. Furthermore, fungi (3 strains), bacteria (4 strains) and actinomycetes (5 strains) constituted 12 strains in total were gained by plate isolation from DM-1.

为了获得具有木质素有效先降解作用的微生物群落，在继代培养后，从腐烂的树干，腐烂的树桩和附近土壤中获得的样品经过筛选和分离。动态变化表明，它们的群落结构受到pH的影响，并在培养6天后趋于稳定。通过连续培养5代以上获得了所需的命名为DM-1的DM-1，在培养16天后观察到了其对木质素降解的高选择性（SV = 2.78）。同时，由于DM-1的生物降解，树木装饰物的纤维结晶度显着降低（10.35％），通过选择性去除木质素显示出更多的纤维素暴露。通过PCR扩增和16S rDNA测序对DM-1的多样性进行了研究，结果表明：中生根瘤菌，纤维素微生物，潘多拉菌，无色杆菌和嗜麦芽激素是主要的属。此外，通过从DM-1中分离板，获得了总共12个菌株的真菌（3个菌株），细菌（4个菌株）和放线菌（5个菌株）。