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Cancer Cell Membrane-Biomimetic Nanoprobes with Two-Photon Excitation and Near-Infrared Emission for Intravital Tumor Fluorescence Imaging
ACS Nano ( IF 17.1 ) Pub Date : 2018-01-19 00:00:00 , DOI: 10.1021/acsnano.7b07716
Yanlin Lv 1, 2 , Ming Liu 3, 4 , Yong Zhang 3 , Xuefei Wang 1 , Fan Zhang 2 , Feng Li 2 , Wei-Er Bao 2 , Jie Wang 1 , Yuanlin Zhang 1 , Wei Wei 2 , Guanghui Ma 2 , Liancheng Zhao 3 , Zhiyuan Tian 1
Affiliation  

Biomimetic fluorescent nanoprobes capable of emitting near-infrared (NIR) fluorescence (λmax ≈ 720 nm) upon excitation of 800 nm light were developed. The key conjugated polymer enabled two-photon absorption and Förster resonance energy transfer (FRET) processes within the nanoprobes, which imparted the nanoprobes with ideal NIR-incoming-NIR-outgoing fluorescence features. The cancer cell membrane (CM) coating endowed these nanoprobes with perfect biocompatibility and highly specific targeting ability to homologous tumors. It was believed that CM encapsulation provided an additional protecting layer for the photoactive components residing in the core of nanoprobes for retaining their intrinsic fluorescing ability in the physiological milieu. The long-term structural integrity, excellent photostability (fluorescence decrease <10% upon 30 min illumination of 800 nm pulse laser), high NIR fluorescence quantum yield (∼20%), and long in vivo circulation time of the target nanoprobes were also confirmed. The ability of these feature-packed nanoprobes for circumventing the challenges of absorption and light scattering caused by cellular structures and tissues was definitely confirmed via in vivo and in vitro experiments. The superior performances of these nanoprobes in terms of fluorescence signaling as well as targeting specificity were verified in intravital fluorescence imaging on tumor-bearing model mice. Specifically, these nanoprobes unequivocally enabled high-resolution visualization of the fine heterogeneous architectures of intravital tumor tissue, which proclaims the great potential of this type of probe for high-contrast fluorescence detection of thick biological samples in practical applications.

中文翻译:

双光子激发和近红外发射的癌细胞膜仿生纳米探针用于体内肿瘤荧光成像

能够发射近红外(NIR)荧光的仿生荧光纳米探针(最大λ激发了800 nm的光后≈720 nm(≈720 nm)。关键的共轭聚合物能够在纳米探针中实现双光子吸收和Förster共振能量转移(FRET)过程,从而赋予纳米探针理想的NIR传入-NIR传出荧光特性。癌细胞膜(CM)涂层使这些纳米探针具有完美的生物相容性和对同源肿瘤的高度特异性靶向能力。据信,CM包封为存在于纳米探针核心中的光敏组分提供了额外的保护层,以在生理环境中保留其固有的发荧光能力。长期的结构完整性,出色的光稳定性(在800 nm脉冲激光照射30分钟后,荧光强度降低<10%),高NIR荧光量子产率(〜20%)和长寿命还确认了目标纳米探针的体内循环时间。这些功能丰富的纳米探针克服了细胞结构和组织引起的吸收和光散射挑战的能力已通过体内体外明确证实实验。这些纳米探针在荧光信号以及靶向特异性方面的优越性能已在荷瘤模型小鼠的活体荧光成像中得到验证。具体而言,这些纳米探针明确地实现了活体肿瘤组织精细异质结构的高分辨率可视化,这宣告了这种类型的探针在实际应用中对厚生物样品进行高对比度荧光检测的巨大潜力。
更新日期:2018-01-19
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