Chrysin (5,7-dihydroxyflavone) is a natural and biologically active compound which has many biological activities as an anticancer agent. The current report is aimed at finding out whether the antitumor potential of chrysin, evidenced in vitro and in vivo, is linked or not to its effect on immunological mechanisms of melanoma-bearing mice.

Chrysin-treated B16F10 cells were analyzed for their metabolic rate and apoptotic potentials. In vivo, BALB/c mice received a subcutaneous injection of B16F10 melanoma cells prior to antitumor treatments with chrysin (50 mg/kg b.w) for 14 days and 21 days.

The results showed that chrysin inhibited cancer cell growth at a dose-dependent manner by inducing apoptosis and cell cycle arrest at G2/M phase. Moreover, chrysin suppressed melanoma tumor growth at an average of 60% (after 14 days of treatment) and 71% (after 21 days of treatment) compared to the tumor-bearing group. Furthermore, chrysin treatment increased the cytotoxic activity of NK, CTL and macrophages.

The findings showed that chrysin antitumor action on the murine melanoma model was very promising, suggesting that chrysin could be a potentially good candidate for future use in alternative anti-melanoma treatments.

菊花（5,7-二羟基黄酮）是一种天然的生物活性化合物，具有许多生物活性作为抗癌剂。本报告旨在发现在体外和体内证明的菊花蛋白的抗肿瘤潜力是否与其对荷黑素瘤小鼠免疫机制的影响有关。

分析了用Chrysin处理的B16F10细胞的代谢率和凋亡潜能。在体内，BALB / c小鼠接受了皮下注射B16F10黑色素瘤细胞的治疗，之后用菊花蛋白（50 mg / kg bw）进行了14天和21天的抗肿瘤治疗。

结果表明，chrysin通过诱导细胞凋亡和细胞周期阻滞在G2 / M期，以剂量依赖的方式抑制癌细胞的生长。此外，相比于荷瘤组，Chrysin抑制黑素瘤肿瘤的生长平均为60％（治疗14天后）和71％（治疗21天后）。此外，菊花素处理增加了NK，CTL和巨噬细胞的细胞毒性活性。

研究结果表明，菊花素对小鼠黑色素瘤模型的抗肿瘤作用非常有前途，这表明菊花素可能是将来在替代性抗黑色素瘤治疗中的潜在潜在候选药物。