A novel water-soluble fluorescein-based fluorescent probe for superoxide detection was developed. The probe is fairly stable under neutral and acidic conditions. It can be used to detect superoxide both in solution with the detection limit of 2.2 μM and in living cells. Cell imaging experiments indicated that such a probe displayed good cell penetration and O₂^•– could be detected with PMA-stimulated HepG2 cells in both mitochondria and lysosome. Such a probe is the first dual mitochondria- and lysosome- targetable fluorescent chemodosimeter. Additionally, O₂^•– in intact live zebrafish embryos was successfully visualized under PMA-stimulated conditions, and the possible detection mechanism was studied as well.

中文翻译：

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具有双重线粒体/溶酶体靶向性的水溶性荧光探针，用于在活细胞和斑马鱼胚胎中进行选择性超氧化物检测

开发了一种新型的用于超氧化物检测的水溶性荧光素基荧光探针。该探针在中性和酸性条件下相当稳定。它可以用于检测极限浓度为2.2μM的溶液中以及活细胞中的超氧化物。细胞成像实验表明，这种探针显示出良好的细胞渗透性，并且可以通过PMA刺激的线粒体和溶酶体中的HepG2细胞检测到O ₂ ^•–。这样的探针是第一个双重线粒体和溶酶体可靶向的荧光化学计量仪。此外，在PMA刺激的条件下，成功地观察了完整的活斑马鱼胚胎中的O ₂ ^•–，并研究了可能的检测机制。