The aim of this study was to investigate the possibility of improving anaerobic fermentation of carbon monoxide (CO), a dominant component in syngas, through the assistance of different carbon materials (activated carbon, biochar, graphene, carbon cloth, and carbon fiber). Results showed that only biochar and activated carbon could promote CO utilization when the p_CO increased from 0.07 to 0.21 atm and promoted CO methanization but was delayed until the p_CO further increased from 0.21 to 0.41 atm. Compared to the control without carbon material addition, the CO conversion rate increased by up to 149% and 193% and the methane (CH₄) production rate was up to 238% and 186%, respectively, for the biochar and activated carbon scenarios. Graphene and carbon cloth did not accelerate CO utilization but could stabilize the bioreaction at a high p_CO. Natural stable carbon isotope signals revealed that CH₄ production from CO was mainly via acetate at low p_CO and via hydrogen at high p_CO. Results from high-throughput sequencing showed that biochar and activated carbon boosted the growth of Rikenellaceae and Thiobacillus in the solid phase. Synergistaceae which can be in coculture with Methanobacterium was boosted in the liquid phase in biochar group and activated carbon group.

中文翻译：

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开发碳材料以加速启动和增强CO厌氧开放培养发酵的过程稳定性

这项研究的目的是研究通过不同碳材料（活性炭，生物炭，石墨烯，碳布和碳纤维）的协助来改善合成气中主要成分一氧化碳（CO）的厌氧发酵的可能性。结果表明，当p _CO从0.07 atm增加到0.21 atm并促进CO甲烷化时，只有生物炭和活性炭可以提高CO利用率，但是被延迟到p _CO从0.21 atm进一步增加到0.41 atm为止。与不添加碳材料的对照相比，CO转化率分别提高了149％和193％，甲烷（CH ₄生物炭和活性炭方案的生产率分别高达238％和186％。石墨烯，碳布没有加速CO利用率，但可以在一个高稳定的生物反应p _CO。自然稳定的碳同位素的信号表明，CH ₄从CO生产，主要是通过在醋酸低p _CO，并通过在高氢气p _CO。高通量测序的结果表明，生物炭和活性炭在固相中促进了Rikenellaceae和Thiobacillus的生长。在生物炭组和活性炭组中，可以与甲烷菌共培养的增效科在液相中得到增强。